DIAPH3 Antibody - #DF12197
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12197, RRID:AB_2845002.
折りたたみ／展開
AN; AUNA1; Dia2; diap3; DIAP3_HUMAN; DIAPH3; Diaphanous homolog 3 (Drosophila); Diaphanous homolog 3; Diaphanous related formin 3; Diaphanous, Drosophila, homolog of, 3; Diaphanous-related formin-3; DKFZp434C0931; DKFZp686A13178; DRF3; FLJ34705; mDia2; NSDAN; OTTHUMP00000018480; Protein diaphanous homolog 3; RP11-26P21.1;
免疫原
- Q9NSV4 DIAP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERHQPRLHHPAQGSAAGTPYPSSASLRGCRESKMPRRKGPQHPPPPSGPEEPGEKRPKFHLNIRTLTDDMLDKFASIRIPGSKKERPPLPNLKTAFASSDCSAAPLEMMENFPKPLSENELLELFEKMMEDMNLNEDKKAPLREKDFSIKKEMVMQYINTASKTGSLKRSRQISPQEFIHELKMGSADERLVTCLESLRVSLTSNPVSWVESFGHEGLGLLLDILEKLISGKIQEKVVKKNQHKVIQCLKALMNTQYGLERIMSEERSLSLLAKAVDPRHPNMMTDVVKLLSAVCIVGEESILEEVLEALTSAGEEKKIDRFFCIVEGLRHNSVQLQVACMQLINALVTSPDDLDFRLHIRNEFMRCGLKEILPNLKCIKNDGLDIQLKVFDEHKEEDLFELSHRLEDIRAELDEAYDVYNMVWSTVKETRAEGYFISILQHLLLIRNDYFIRQQYFKLIDECVSQIVLHRDGMDPDFTYRKRLDLDLTQFVDICIDQAKLEEFEEKASELYKKFEKEFTDHQETQAELQKKEAKINELQAELQAFKSQFGALPADCNIPLPPSKEGGTGHSALPPPPPLPSGGGVPPPPPPPPPPPLPGMRMPFSGPVPPPPPLGFLGGQNSPPLPILPFGLKPKKEFKPEISMRRLNWLKIRPHEMTENCFWIKVNENKYENVDLLCKLENTFCCQQKERREEEDIEEKKSIKKKIKELKFLDSKIAQNLSIFLSSFRVPYEEIRMMILEVDETRLAESMIQNLIKHLPDQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEKYPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKVCNHENQKVQLTEGSRSHYNINCNSTRTPVAKELNYNLDTHTSTGRIKAAEKKEACNVESNRKKETELLGSFSKNESVPEVEALLARLRAL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NSV4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R7 | Methylation | Uniprot | |
| T19 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S23 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| R28 | Methylation | Uniprot | |
| R31 | Methylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K94 | Methylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| K518 | Ubiquitination | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| K536 | Ubiquitination | Uniprot | |
| K548 | Ubiquitination | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| K653 | Methylation | Uniprot | |
| K672 | Ubiquitination | Uniprot | |
| Y673 | Phosphorylation | Uniprot | |
| K681 | Ubiquitination | Uniprot | |
| K691 | Ubiquitination | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| S717 | Phosphorylation | Uniprot | |
| K718 | Ubiquitination | Uniprot | |
| S728 | Phosphorylation | Uniprot | |
| S729 | Phosphorylation | Uniprot | |
| K759 | Ubiquitination | Uniprot | |
| K874 | Ubiquitination | Uniprot | |
| K909 | Ubiquitination | Uniprot | |
| K912 | Ubiquitination | Uniprot | |
| K920 | Ubiquitination | Uniprot | |
| K934 | Ubiquitination | Uniprot | |
| K948 | Ubiquitination | Uniprot | |
| K961 | Ubiquitination | Uniprot | |
| K991 | Ubiquitination | Uniprot | |
| K1032 | Ubiquitination | Uniprot | |
| K1053 | Ubiquitination | Uniprot | |
| S1091 | Phosphorylation | Uniprot | |
| S1093 | Phosphorylation | Uniprot | |
| S1096 | Phosphorylation | Uniprot | |
| K1110 | Ubiquitination | Uniprot | |
| K1134 | Ubiquitination | Uniprot | |
| Y1138 | Phosphorylation | Uniprot | |
| S1145 | Phosphorylation | Uniprot | |
| S1162 | Phosphorylation | Uniprot | |
| K1166 | Ubiquitination | Uniprot | |
| K1176 | Ubiquitination | Uniprot | |
| S1179 | Phosphorylation | Uniprot |
研究背景
Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.
Ubiquitinated.
Cytoplasm. Nucleus.
Note: During mitosis, co-localizes with the actin-rich cleavage furrow and with the microtubule-rich central spindle during cytokinesis (PubMed:18755006, PubMed:19457867). Shuttles between the cytoplasm and the nucleus (By similarity).
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.
Belongs to the formin homology family. Diaphanous subfamily.
研究領域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
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