SNAPIN Antibody - #DF12230
| 製品: | SNAPIN Antibody |
| カタログ: | DF12230 |
| タンパク質の説明: | Rabbit polyclonal antibody to SNAPIN |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Rabbit, Dog |
| 分子量: | 15-18 kDa; 15kD(Calculated). |
| ユニプロット: | O95295 |
| RRID: | AB_2845035 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12230, RRID:AB_2845035.
折りたたみ／展開
OTTHUMP00000035157; SNAP associated protein; SNAP-25-binding protein; SNAP-associated protein; SNAP25BP; SNAPAP; Snapin; SNAPN_HUMAN; SNARE associated protein snapin; SNARE-associated protein Snapin; Synaptosomal associated protein 25 binding protein; Synaptosomal-associated protein 25-binding protein;
免疫原
- O95295 SNAPN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGAGSAAVSGAGTPVAGPTGRDLFAEGLLEFLRPAVQQLDSHVHAVRESQVELREQIDNLATELCRINEDQKVALDLDPYVKKLLNARRRVVLVNNILQNAQERLRRLNHSVAKETARRRAMLDSGIYPPGSPGK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95295 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| S50 | O-Glycosylation | Uniprot | |
| S50 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K73 | Ubiquitination | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| T117 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| S126 | Phosphorylation | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K136 | Ubiquitination | Uniprot |
研究背景
Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells. As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor.
Phosphorylated by CSNK1D/CK1 (By similarity). Phosphorylated by PKD, phosphorylation controls SNAPIN protein stability.
Membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytosol. Cytoplasm>Perinuclear region. Golgi apparatus membrane. Lysosome membrane. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane.
Note: Colocalizes with NANOS1 and PUM2 in the perinuclear region of germ cells.
Expressed in male germ cells of adult testis (at protein level).
Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos. Component of the BLOC-one-related complex (BORC) which is composed of BLOC1S1, BLOC1S2, BORCS5, BORCS6, BORCS7, BORCS8, KXD1 and SNAPIN. Associates with the SNARE complex. Interacts with CSNK1D, SNAP23 and STX4A but not with STX1A, VAMP2 and SYT1. Interacts with SNAP25; the interaction with SNAP25 is increased by its phosphorylation. Interacts with CNTRL, NANOS1, PUM2 and RGS7. Interacts with TOR1A; the interaction is direct and associates SNAPIN with the CSN complex.
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL70.
Belongs to the SNAPIN family.
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