DNAJC10 Antibody - #DF12269
| 製品: | DNAJC10 Antibody |
| カタログ: | DF12269 |
| タンパク質の説明: | Rabbit polyclonal antibody to DNAJC10 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 75-80 kDa; 91kD(Calculated). |
| ユニプロット: | Q8IXB1 |
| RRID: | AB_2845074 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12269, RRID:AB_2845074.
折りたたみ／展開
DKFZp434J1813; DnaJ (Hsp40) homolog subfamily C member 10; DnaJ homolog subfamily C member 10; DNAJC 10; ER resident protein ERdj5; ERdj 5; ERdj5; J domain containing protein disulfide isomerase like protein; JPDI; Macrothioredoxin; MGC104194; MTHr;
免疫原
- Q8IXB1 DJC10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGVWLNKDDYIRDLKRIILCFLIVYMAILVGTDQDFYSLLGVSKTASSREIRQAFKKLALKLHPDKNPNNPNAHGDFLKINRAYEVLKDEDLRKKYDKYGEKGLEDNQGGQYESWNYYRYDFGIYDDDPEIITLERREFDAAVNSGELWFVNFYSPGCSHCHDLAPTWRDFAKEVDGLLRIGAVNCGDDRMLCRMKGVNSYPSLFIFRSGMAPVKYHGDRSKESLVSFAMQHVRSTVTELWTGNFVNSIQTAFAAGIGWLITFCSKGGDCLTSQTRLRLSGMLDGLVNVGWMDCATQDNLCKSLDITTSTTAYFPPGATLNNKEKNSILFLNSLDAKEIYLEVIHNLPDFELLSANTLEDRLAHHRWLLFFHFGKNENSNDPELKKLKTLLKNDHIQVGRFDCSSAPDICSNLYVFQPSLAVFKGQGTKEYEIHHGKKILYDILAFAKESVNSHVTTLGPQNFPANDKEPWLVDFFAPWCPPCRALLPELRRASNLLYGQLKFGTLDCTVHEGLCNMYNIQAYPTTVVFNQSNIHEYEGHHSAEQILEFIEDLMNPSVVSLTPTTFNELVTQRKHNEVWMVDFYSPWCHPCQVLMPEWKRMARTLTGLINVGSIDCQQYHSFCAQENVQRYPEIRFFPPKSNKAYHYHSYNGWNRDAYSLRIWGLGFLPQVSTDLTPQTFSEKVLQGKNHWVIDFYAPWCGPCQNFAPEFELLARMIKGKVKAGKVDCQAYAQTCQKAGIRAYPTVKFYFYERAKRNFQEEQINTRDAKAIAALISEKLETLRNQGKRNKDEL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IXB1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K66 | Ubiquitination | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| T133 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K640 | Acetylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| T673 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| K778 | Ubiquitination | Uniprot | |
| R783 | Methylation | Uniprot |
研究背景
Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.
Endoplasmic reticulum lumen.
Interacts with EDEM1 (By similarity). Interacts with HSPA5 (via its J domain).
The thioredoxin-like regions Trxb 1 and 2 lack a redox-active CXXC motif.
Thioredoxin domains 3 and 4 are the primary reductase domains.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.