製品: Hemoglobin epsilon Antibody
カタログ: DF12277
タンパク質の説明: Rabbit polyclonal antibody to Hemoglobin epsilon
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Rabbit
分子量: 16 kDa; 16kD(Calculated).
ユニプロット: P02100
RRID: AB_2845082

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(82%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
Hemoglobin epsilon Antibody detects endogenous levels of total Hemoglobin epsilon.
RRID:
AB_2845082
引用形式: Affinity Biosciences Cat# DF12277, RRID:AB_2845082.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Epsilon-globin; HBE_HUMAN; HBE1; Hemoglobin epsilon chain; Hemoglobin subunit epsilon;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P02100 HBE_HUMAN:

Red blood cells.

タンパク質配列:
MVHFTAEEKAAVTSLWSKMNVEEAGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPKVKAHGKKVLTSFGDAIKNMDNLKPAFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFGKEFTPEVQAAWQKLVSAVAIALAHKYH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
91
Bovine
82
Chicken
64
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02100 基板として

Site PTM Type Enzyme
S14 Phosphorylation
S17 Phosphorylation
K18 Ubiquitination
Y36 Phosphorylation
T39 Phosphorylation
S50 Phosphorylation
S51 Phosphorylation
K60 Acetylation
K60 Ubiquitination
K66 Acetylation
K67 Ubiquitination
S71 Phosphorylation
K77 Acetylation
K77 Ubiquitination
K83 Acetylation
K83 Ubiquitination
K145 Acetylation

研究背景

機能:

The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin.

組織特異性:

Red blood cells.

サブユニット構造:

Heterotetramer of two alpha chains and two epsilon chains in early embryonic hemoglobin Gower-2; two zeta chains and two epsilon chains in early embryonic hemoglobin Gower-1.

タンパク質ファミリー:

Belongs to the globin family.

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