HOMER1 Antibody - #DF12280
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12280, RRID:AB_2845085.
折りたたみ／展開
HOME1_HUMAN; Homer; HOMER 1A; HOMER 1B; HOMER 1C; Homer homolog 1 (Drosophila); homer homolog 1; Homer protein homolog 1; homer scaffolding protein 1; Homer, Drosophila, homolog of, 1; Homer, neuronal immediate early gene, 1; Homer-1; Homer1; HOMER1A; HOMER1B; HOMER1C; SYN47; Ves 1;
免疫原
- Q86YM7 HOME1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGEQPIFSTRAHVFQIDPNTKKNWVPTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNMTFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFAEKFQEFKEAARLAKEKSQEKMELTSTPSQESAGGDLQSPLTPESINGTDDERTPDVTQNSEPRAEPTQNALPFSHSSAISKHWEAELATLKGNNAKLTAALLESTANVKQWKQQLAAYQEEAERLHKRVTELECVSSQANAVHTHKTELNQTIQELEETLKLKEEEIERLKQEIDNARELQEQRDSLTQKLQEVEIRNKDLEGQLSDLEQRLEKSQNEQEAFRNNLKTLLEILDGKIFELTELRDNLAKLLECS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86YM7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Acetylation | Uniprot | |
| K73 | Ubiquitination | Uniprot |
研究背景
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA.
Cytoplasm. Cell junction>Synapse>Postsynaptic density. Cell junction>Synapse. Cell projection>Dendritic spine.
Note: Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
Tetramer; this tetrameric structure is critical for forming the high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Interacts with GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2 and SHANK3. Interacts with IFT57 and OPHN1. Isoform 1 encodes a coiled-coil structure that mediates homo- and heteromultimerization. Interacts with SHANK1; forms high-order polymerized complex with a mesh-like network structure, at least composed of SHANK1, HOMER1 and DLGAP1; the complex formation is SHANK1 multimerization dependent.Interacts with SHANK1; forms high-order polymerized complex with a mesh-like network structure, at least composed of SHANK1, HOMER1 and DLGAP1; the complex formation is SHANK1 multimerization dependent (By similarity). Interacts with NFATC4.
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3. The coiled-Coil domain forms an antiparallel tetrameric arrangement (By similarity).
Belongs to the Homer family.
研究領域
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Glutamatergic synapse.
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