Mast Cell Chymase Antibody - #DF12290

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製品: Mast Cell Chymase Antibody
カタログ: DF12290
タンパク質の説明: Rabbit polyclonal antibody to Mast Cell Chymase
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Dog
分子量: 27-30 kDa; 27kD(Calculated).
ユニプロット: P23946
RRID: AB_2845095

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Dog(83%)
クローナリティ:
Polyclonal
特異性:
Mast Cell Chymase Antibody detects endogenous levels of total Mast Cell Chymase.
RRID:
AB_2845095
引用形式: Affinity Biosciences Cat# DF12290, RRID:AB_2845095.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Alpha-chymase; Chymase 1; Chymase 1 mast cell; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; Chymase; Chymase, heart; Chymase, mast cell; CMA1; CMA1_HUMAN; CYH; CYM; EC 3.4.21.39; Mast cell chymase 1; Mast cell protease 3; Mast cell protease 5; Mast cell protease I; Mast cell protease III; Mcp-5; MCP3P; Mcpt5; MCT1; MGC119890; MGC119891; MMCP-5;

免疫原

免疫原:
Uniprot:

P23946(CMA1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CMA1(1215)
発現特異性:
P23946 CMA1_HUMAN:

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

タンパク質配列:
MLLLPLPLLLFLLCSRAEAGEIIGGTECKPHSRPYMAYLEIVTSNGPSKFCGGFLIRRNFVLTAAHCAGRSITVTLGAHNITEEEDTWQKLEVIKQFRHPKYNTSTLHHDIMLLKLKEKASLTLAVGTLPFPSQFNFVPPGRMCRVAGWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
83
Rabbit
70
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23946 基板として

Site PTM Type Enzyme
S15 Phosphorylation
Y35 Phosphorylation
Y38 Phosphorylation
N80 N-Glycosylation
N103 N-Glycosylation

研究背景

機能:

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

細胞の位置付け:

Secreted. Cytoplasmic granule.
Note: Mast cell granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

タンパク質ファミリー:

Belongs to the peptidase S1 family. Granzyme subfamily.

研究領域

· Organismal Systems > Endocrine system > Renin-angiotensin system.   (View pathway)

参考文献

1). SYNPO2 promotes the development of BLCA by upregulating the infiltration of resting mast cells and increasing the resistance to immunotherapy. Oncology reports, 2024 (PubMed: 38038167) [IF=4.2]

Application: IF/ICC    Species: Mouse    Sample: lung tissue

Figure 5. SYNPO2 expression enhances mast cell infiltration. (A) SYNPO2 expression in 24 types of immune cell. Red text indicates a high correlation. (B) Representative toluidine and immunohistochemical staining in clinical Bladder Urothelial Carcinoma (BLCA) samples with low and high SYNPO2 expression. Purple represents cell degranulation. Scale bar, 100 µm. (C) Mast cell number/field in low and high SYNPO2 expression groups (P=0.0053). Correlation between SYNPO2 and (D) TPSAB1 and (E) CCR3 expression based on H-score. (F) Mouse serum IL-6 levels in NC (n=5) and SY (n=5; P=0.002). (G) Representative indirect immunofluorescence of a frozen lung section from a mouse model with pulmonary metastasis. The black arrow indicates the area of tumor invasion in the lung tissue. (H) Mouse serum TGF-β1 levels (P=0.045). (I) CMA-positive cells (n=3, P=0.0036). SYNPO2, synaptopodin-2; TPSAB1, Tryptase alpha/beta-1; CCR3, C-C chemokine receptor type 3; NC, Negative Control; SY, SYNPO2 overexpression; CMA1, Chymase 1. * and ** indicate P

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