RAP1B Antibody - #DF12309
| 製品: | RAP1B Antibody |
| カタログ: | DF12309 |
| タンパク質の説明: | Rabbit polyclonal antibody to RAP1B |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 21 kDa; 21kD(Calculated). |
| ユニプロット: | P61224 |
| RRID: | AB_2845114 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12309, RRID:AB_2845114.
折りたたみ／展開
2810443E11Rik; DKFZp586H0723; GTP binding protein smg p21B; K-REV; MGC93206; RAL1B; RAP1B; RAP1B member of RAS oncogene family; Ras family small GTP binding protein RAP1B; RAS related protein 1b; Ras related protein Rap 1b; Ras related protein RAP1B;
免疫原
- P61224 RAP1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P61224 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| Y40 | Phosphorylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| Y159 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| S179 | Phosphorylation | P17612 (PRKACA) , Q13976 (PRKG1) | Uniprot |
| S180 | Phosphorylation | Uniprot | |
| C181 | Methylation | Uniprot |
研究背景
GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.
Cell membrane. Cytoplasm>Cytosol. Cell junction.
Note: May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction.
Heterodimer with RAP1GAP. Interacts with EPAC2, SGSM1, SGSM2 and SGSM3. Interacts with KRIT1.
Belongs to the small GTPase superfamily. Ras family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Digestive system > Pancreatic secretion.
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