RNF156 Antibody - #DF12312
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12312, RRID:AB_2845117.
折りたたみ／展開
E3 ubiquitin-protein ligase MGRN1; KIAA0544; Mahogunin ring finger 1; Mahogunin RING finger protein 1; MGRN 1; Mgrn1; MGRN1_HUMAN; Probable E3 ubiquitin protein ligase MGRN1; RING finger protein 156; RNF 156;
免疫原
- O60291 MGRN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEGYLFGENMDLNFLGSRPVQFPYVTPAPHEPVKTLRSLVNIRKDSLRLVRYKDDADSPTEDGDKPRVLYSLEFTFDADARVAITIYCQASEEFLNGRAVYSPKSPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVIQAVVDEGDVVEVTGHAHVLLAAFEKHMDGSFSVKPLKQKQIVDRVSYLLQEIYGIENKNNQETKPSDDENSDNSNECVVCLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLEHDEHSCPFKKSKPHPASLASKKPKRETNSDSVPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSRQKGRPQSKAPDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITEEVDESSSPQQGTRAASIENVLQDSSPEHCGRGPPADIYLPALGPDSCSVGIDE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60291 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| Y100 | Phosphorylation | Uniprot | |
| K101 | Acetylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| Y254 | Phosphorylation | Uniprot | |
| K259 | Acetylation | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| S402 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| Y411 | Phosphorylation | Uniprot | |
| T415 | Phosphorylation | Uniprot | |
| Y416 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| T454 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S523 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot |
研究背景
E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling (By similarity).
Autoubiquitinated in vitro.
Early endosome.
Note: The endosomal localization is dependent on the interaction with TSG101.
Cytoplasm>Cytosol. Nucleus.
Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
Cytoplasm>Cytosol. Nucleus.
Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
Cytoplasm>Cytosol. Cell membrane.
Cytoplasm>Cytosol. Cell membrane.
Interacts with MC1R and MC4R, but not with TBXA2R. Interacts with TSG101. Interacts with mislocalized cytosolically exposed PRNP; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement (By similarity).
The RING finger is required for ubiquitin ligase activity.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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