URAT1 Antibody - #DF12340

(6)   (2)  
製品: URAT1 Antibody
カタログ: DF12340
タンパク質の説明: Rabbit polyclonal antibody to URAT1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat, Pig, Monkey
予測: Pig, Horse
分子量: 40 kDa,65-70 kDa; 60kD(Calculated).
ユニプロット: Q96S37
RRID: AB_2845145

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Pig,Monkey
予測:
Horse(91%)
クローナリティ:
Polyclonal
特異性:
URAT1 Antibody detects endogenous levels of total URAT1.
RRID:
AB_2845145
引用形式: Affinity Biosciences Cat# DF12340, RRID:AB_2845145.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Organic anion transporter 4-like protein; Renal-specific transporter; RST; S22AC_HUMAN; Slc22a12; Solute carrier family 22 member 12; Urate anion exchanger 1;

免疫原

免疫原:
Uniprot:

Q96S37(S22AC_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC22A12(116085)
発現特異性:
Q96S37 S22AC_HUMAN:

Detected in kidney (at protein level). Detected in fetal and adult kidney. Detected in epithelial cells of proximal tubules in renal cortex.

タンパク質配列:
MAFSELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTCISTLCWFAFGFTFFGLALDLQALGSNIFLLQMFIGVVDIPAKMGALLLLSHLGRRPTLAASLLLAGLCILANTLVPHEMGALRSALAVLGLGGVGAAFTCITIYSSELFPTVLRMTAVGLGQMAARGGAILGPLVRLLGVHGPWLPLLVYGTVPVLSGLAALLLPETQSLPLPDTIQDVQNQAVKKATHGTLGNSVLKSTQF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Pig
82
Bovine
73
Dog
73
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Required for efficient urate re-absorption in the kidney. Regulates blood urate levels. Mediates saturable urate uptake by facilitating the exchange of urate against organic anions.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.
Note: Detected in the luminal membrane of the epithelium of renal proximal tubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in kidney (at protein level). Detected in fetal and adult kidney. Detected in epithelial cells of proximal tubules in renal cortex.

サブユニット構造:

Interacts with PDZK1.

タンパク質ファミリー:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

参考文献

1). Caffeic acid phenethyl ester alleviated hypouricemia in hyperuricemic mice through inhibiting XOD and up-regulating OAT3. PHYTOMEDICINE, 2022 (PubMed: 35714456) [IF=7.9]
2). Sonneratia apetala seed oil attenuates potassium oxonate/hypoxanthine-induced hyperuricemia and renal injury in mice. Food & Function, 2021 (PubMed: 34606558) [IF=6.1]
3). The Extract of Sonneratia apetala Leaves and Branches Ameliorates Hyperuricemia in Mice by Regulating Renal Uric Acid Transporters and Suppressing the Activation of the JAK/STAT Signaling Pathway. Frontiers in Pharmacology, 2021 (PubMed: 34483901) [IF=5.6]

Application: WB    Species: Mice    Sample: kidney tissues

FIGURE 10 Effects of SAL on the expression of uric acid transporters. (A) The expression levels of the URAT1, GLUT9, and OAT1; quantitative results of Western blot analyses for (B) GLUT9; (C) URAT1; (D) OAT1. β-actin was used as a loading control. All data are expressed as mean ± SD (n = 3). ## p < 0.01 vs. the intact group, **p < 0.01 vs. the vehicle group.
4). Berberrubine attenuates potassium oxonate- and hypoxanthine-induced hyperuricemia by regulating urate transporters and JAK2/STAT3 signaling pathway. European Journal of Pharmacology, 2021 (PubMed: 34699754) [IF=5.0]
5). Ethanol extract of Nepeta angustifoia C. Y. Wu ameliorates hyperuricemia in fructose-induced mice. Pakistan journal of pharmaceutical sciences, 2022 (PubMed: 36008897) [IF=0.8]
6). Naringenin Attenuates Gout-Induced Renal Injury in Rats Via TLR4/NLRP3 Pathway and Urate Transporters. , 2023

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.