ACAA1 Antibody - #DF12345

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製品: ACAA1 Antibody
カタログ: DF12345
タンパク質の説明: Rabbit polyclonal antibody to ACAA1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 41 kDa; 44kD(Calculated).
ユニプロット: P09110
RRID: AB_2845150

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
ACAA1 Antibody detects endogenous levels of total ACAA1.
RRID:
AB_2845150
引用形式: Affinity Biosciences Cat# DF12345, RRID:AB_2845150.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

3 ketoacyl CoA thiolase peroxisomal; 3-ketoacyl-CoA thiolase; ACAA; ACAA1; Acetyl CoA acyltransferase 1; Acetyl Coenzyme A acyltransferase 1; Acetyl-CoA acyltransferase; Acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3 oxoacyl Coenzyme A; Beta ketothiolase; Beta-ketothiolase; Peroxisomal 3 oxoacyl CoA thiolase; Peroxisomal 3 oxoacyl Coenzyme A thiolase; Peroxisomal 3-oxoacyl-CoA thiolase; peroxisomal; PTHIO; THIK_HUMAN; THIO;

免疫原

免疫原:
Uniprot:

P09110(THIK_HUMAN) >>Visit HPA database.

遺伝子(ID):
ACAA1(30)
タンパク質配列:
MQRLQVVLGHLRGPADSGWMPQAAPCLSGAPQASAADVVVVHGRRTAICRAGRGGFKDTTPDELLSAVMTAVLKDVNLRPEQLGDICVGNVLQPGAGAIMARIAQFLSDIPETVPLSTVNRQCSSGLQAVASIAGGIRNGSYDIGMACGVESMSLADRGNPGNITSRLMEKEKARDCLIPMGITSENVAERFGISREKQDTFALASQQKAARAQSKGCFQAEIVPVTTTVHDDKGTKRSITVTQDEGIRPSTTMEGLAKLKPAFKKDGSTTAGNSSQVSDGAAAILLARRSKAEELGLPILGVLRSYAVVGVPPDIMGIGPAYAIPVALQKAGLTVSDVDIFEINEAFASQAAYCVEKLRLPPEKVNPLGGAVALGHPLGCTGARQVITLLNELKRRGKRAYGVVSMCIGTGMGAAAVFEYPGN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
92
Xenopus
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09110 基板として

Site PTM Type Enzyme
T59 Phosphorylation
T60 Phosphorylation
T70 Phosphorylation
S124 Phosphorylation
S125 Phosphorylation
S132 Phosphorylation
S141 Phosphorylation
S166 Phosphorylation
K198 Acetylation
K198 Ubiquitination
K209 Ubiquitination
K234 Acetylation
K237 Acetylation
S251 Phosphorylation
T252 Phosphorylation
K259 Acetylation
S269 Phosphorylation
S291 Phosphorylation
K292 Ubiquitination
Y323 Phosphorylation
S337 Phosphorylation
T389 Phosphorylation
K395 Acetylation

研究背景

細胞の位置付け:

Peroxisome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer.

タンパク質ファミリー:

Belongs to the thiolase-like superfamily. Thiolase family.

研究領域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Lipid metabolism > alpha-Linolenic acid metabolism.

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

参考文献

1). Transcriptome and proteomics conjoint analysis reveal anti-alcoholic liver injury effect of Dianhong Black Tea volatile substances. Food science & nutrition, 2024 (PubMed: 38268900) [IF=3.9]

Application: WB    Species: Rat    Sample:

FIGURE 5 DBTVS decreased the expression of FADS2, CD36, and ACAA1. (a) Representative blots of FADS2, CYP7A1, CD36, ACSL1, ACAA1, and HMGCS2. (b) The protein expression levels of FADS2 between different groups. (c) The protein expression levels of CYP7A1 between different groups. (d) The protein expression levels of CD36 between different groups. (e) The protein expression levels of ACSL1 between different groups. (f) The protein expression levels of ACAA1 between different groups. (g) The protein expression levels of HMGCS2 between different groups. Data were presented as mean ± SD. Multiple groups were tested by one‐way ANOVA followed by Dunnett‐t test for all statistical analyses. *p 

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