製品: DCTN6 Antibody
カタログ: DF12381
タンパク質の説明: Rabbit polyclonal antibody to DCTN6
アプリケーション: WB IHC IF/ICC
反応性: Human, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 27 kDa; 21kD(Calculated).
ユニプロット: O00399
RRID: AB_2845186

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(83%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
DCTN6 Antibody detects endogenous levels of total DCTN6.
RRID:
AB_2845186
引用形式: Affinity Biosciences Cat# DF12381, RRID:AB_2845186.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

DCTN6; DCTN6_HUMAN; Dynactin 6; Dynactin subunit 6; Dynactin subunit p27; Novel RGD containing protein; p27; Protein WS-3; Protein WS3; WS 3; WS3;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O00399 DCTN6_HUMAN:

Ubiquitous.

タンパク質配列:
MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVIGEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMKMGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLRRVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
92
Sheep
92
Dog
92
Rabbit
92
Pig
83
Xenopus
63
Zebrafish
50
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00399 基板として

Site PTM Type Enzyme
K110 Ubiquitination
K178 Ubiquitination
T186 Phosphorylation P06493 (CDK1)

研究背景

PTMs:

Phosphorylation at Thr-186 by CDK1 during mitotic prometaphase creates a binding site for PLK1 that facilitates its recruitment to kinetochores.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Chromosome>Centromere>Kinetochore.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

サブユニット構造:

Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10. Within the complex DCTN6 forms a heterodimer with DCTN5. Interacts with PLK1.

タンパク質ファミリー:

Belongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.

研究領域

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

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