製品: EIF2B1 Antibody
カタログ: DF12392
タンパク質の説明: Rabbit polyclonal antibody to EIF2B1
アプリケーション: WB IHC
反応性: Human
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 34 kDa; 34kD(Calculated).
ユニプロット: Q14232
RRID: AB_2845197

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(92%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
EIF2B1 Antibody detects endogenous levels of total EIF2B1.
RRID:
AB_2845197
引用形式: Affinity Biosciences Cat# DF12392, RRID:AB_2845197.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

EI2BA_HUMAN; eIF-2B GDP-GTP exchange factor subunit alpha; EIF2B; Eif2b1; EIF2BA; Eukaryotic translation initiation factor 2B subunit 1 alpha 26kDa; Eukaryotic translation initiation factor 2B subunit alpha; Translation initiation factor eIF-2B subunit alpha;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDELIKLYL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Rabbit
100
Dog
92
Chicken
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14232 基板として

Site PTM Type Enzyme
K11 Ubiquitination
K15 Ubiquitination
T29 Phosphorylation
K35 Acetylation
K35 Ubiquitination
K38 Ubiquitination
Y83 Phosphorylation
S84 Phosphorylation
S105 Phosphorylation
S107 Phosphorylation
Y130 Phosphorylation
K145 Methylation
K145 Ubiquitination
S149 Phosphorylation
K163 Acetylation
K253 Ubiquitination
K258 Ubiquitination

研究背景

機能:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

サブユニット構造:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.

タンパク質ファミリー:

Belongs to the eIF-2B alpha/beta/delta subunits family.

研究領域

· Genetic Information Processing > Translation > RNA transport.

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