FBXW7 Antibody - #DF12400
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12400, RRID:AB_2845205.
折りたたみ／展開
AGO; Archipelago; Archipelago homolog; Archipelago, Drosophila, homolog of; CDC4; CDC4, S.cerevisiae, homolog of; DKFZp686F23254; F box and WD 40 domain protein 7 (archipelago homolog, Drosophila); F box and WD 40 domain protein 7; F box and WD repeat domain containing 7; F box protein FBW7; F box protein FBX30; F box protein SEL 10; F box protein SEL10; F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase; F-box and WD-40 domain-containing protein 7; F-box protein FBX30; F-box/WD repeat-containing protein 7; FBW6; FBW7; FBX30; FBXO30; FBXW6; FBXW7; FBXW7_HUMAN; FLJ16457; hAgo; hCdc4; Homolog of C elegans sel 10; Homolog of C.elegans sel10; SEL 10; SEL-10; SEL10;
免疫原
- Q969H0 FBXW7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNQELLSVGSKRRRTGGSLRGNPSSSQVDEEQMNRVVEEEQQQQLRQQEEEHTARNGEVVGVEPRPGGQNDSQQGQLEENNNRFISVDEDSSGNQEEQEEDEEHAGEQDEEDEEEEEMDQESDDFDQSDDSSREDEHTHTNSVTNSSSIVDLPVHQLSSPFYTKTTKMKRKLDHGSEVRSFSLGKKPCKVSEYTSTTGLVPCSATPTTFGDLRAANGQGQQRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKRRKVIKPGFIHSPWKSAYIRQHRIDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q969H0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | P17252 (PRKCA) , P41743 (PRKCI) | Uniprot |
| S18 | Phosphorylation | P41743 (PRKCI) , P17252 (PRKCA) | Uniprot |
| S25 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| S26 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S176 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| T205 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S227 | Phosphorylation | O00141 (SGK1) | Uniprot |
| S349 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| S372 | Phosphorylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| T680 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| T692 | Phosphorylation | Uniprot | |
| T695 | Phosphorylation | Uniprot |
研究背景
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1, and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation. SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (By similarity). Involved in bone homeostasis and negative regulation of osteoclast differentiation. Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination.
Phosphorylation at Thr-205 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation.
Ubiquitinated: autoubiquitinates following phosphorylation at Thr-205 and subsequent interaction with PIN1. Ubiquitination leads to its proteasomal degradation.
Nucleus>Nucleoplasm.
Cytoplasm.
Nucleus>Nucleolus.
Isoform 1 is widely expressed. Isoform 3 is expressed in brain.
Homodimer; homodimerization plays a role in substrate binding and/or ubiquitination and degradation. Component of the SCF(FBXW7) complex consisting of CUL1, RBX1, SKP1 and FBXW7. Interacts (via F-box domain) with SKP1. Interacts (via F-box domain) with pseudophosphatase STYX; the interaction is direct and prevents FBXW7 interaction with SKP1. Interacts with cyclin-E (CCNE1 or CCNE2). Interacts with PSEN1. Forms a trimeric complex with NOTCH1 and SGK1. Interacts with NOTCH1 intracellular domain/NICD and NOTCH4 intracellular domain/NICD. Interacts with NOTCH2 intracellular domain (N2ICD). Interacts with MYC (when phosphorylated). Interacts with USP28, leading to counteract ubiquitination of MYC. Interacts with JUN. Found in a complex with JUN and PRR7. Interacts with JUN and PRR7; the interaction inhibits ubiquitination-mediated JUN degradation promoting its phosphorylation and transcriptional activity. Interacts (when phosphorylated at Thr-205) with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation. Interacts with UBE2QL1. Interacts with FAM83D; promotes FBXW7 degradation. Interacts with MYCN; FBXW7 competes with AURKA for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN. Interacts with STOML1. Interacts with NFE2L1 (By similarity). Interacts with USP36, leading to counteract ubiquitination of MYC. Interacts with NR1D1.
(Microbial infection) Interacts (via WD repeats) with SV40 large T antigen (via CPD region).
The WD repeats mediate interaction with substrates of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.
The F-box domain mediates interaction with SKP1.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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