FXR1 Antibody - #DF12402

FIGURE 5 |Effects of quercetin on Bile acid and farnesoid X receptor 1 (FXR1)/Takeda G‐protein‐coupled receptor 5 (TGR5) axis of db/db mice.(a) Levels of total bile acids (TBAs) in serum and liver in different groups. (b) The relative protein levels of FXR1 and TGR5 in liver of mice normalized to β‐actin as a loading control. Each bar represents the mean ± SEM for groups of six. *p < .05, **p < .01, compared with db/m mice as indicated; #p < .05, ##p < .01, compared with db/db mice as indicated

Figure 4. Cellular uptake of Cy5-labeled NPs in 4T1 cells and fluorescence distribution of Cy5-labeled NPs in 4T1 tumor spheroids. (A) Cellular uptake of Cy5-labeled NPs in 4T1 cells after 2 or 4 h treatment. The CLSM images of 4T1 cells incubated with Cy5 (1 μg/mL, red), Cy5/Oxi-αCD NPs or Cy5/FA-Oxi-αCD NPs (containing 1 μg/mL of Cy5) at 37 °C for 2 or 4 h. Cell nuclei were stained with DAPI (blue). Scale bar represents 20 μm. (B) The semi-quantitative analysis of the corresponding Cy5 fluorescence intensity of intracellular NPs (red) is in Figure 4(A). (C) Cellular uptake of Cy5-labeled NPs in 4T1 cells with or without FR antibody treatment. (D) The semi-quantitative analysis of the corresponding Cy5 fluorescence intensity of intracellular NPs (red) in Figure 4(C). (E) Fluorescence distribution of Cy5-labeled NPs (red) in 4T1 tumor spheroids. Tumor spheroid sections were observed at given time points by CLSM. Cell nuclei were stained with DAPI (blue). Scale bar represents 20 μm for 2 h and 50 μm for 4 h. (F) Semi-quantitative analysis of the corresponding Cy5 fluorescence intensity of NPs (red) in tumor spheroid sections. *, statistically different at p