GSTM3 Antibody - #DF12408

(2)   (2)  
製品: GSTM3 Antibody
カタログ: DF12408
タンパク質の説明: Rabbit polyclonal antibody to GSTM3
アプリケーション: WB IHC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 27-29 kDa; 27kD(Calculated).
ユニプロット: P21266
RRID: AB_2845213

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(80%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
GSTM3 Antibody detects endogenous levels of total GSTM3.
RRID:
AB_2845213
引用形式: Affinity Biosciences Cat# DF12408, RRID:AB_2845213.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

brain GST; brain type mu glutathione S transferase; glutathione S alkyltransferase M3; glutathione S aryltransferase M3; glutathione S transferase M3 (brain); glutathione S transferase M3; Glutathione S transferase Mu 3; glutathione S transferase, Mu 3; Glutathione S-transferase Mu 3; GST class mu 3; GST class-mu 3; GST5; GSTB; Gstm3; GSTM3-3; GSTM3_HUMAN; GTM3; hGSTM3-3; MGC3310; MGC3704; S (hydroxyalkyl)glutathione lyase M3;

免疫原

免疫原:
Uniprot:

P21266(GSTM3_HUMAN) >>Visit HPA database.

遺伝子(ID):
GSTM3(2947)
発現特異性:
P21266 GSTM3_HUMAN:

Testis and brain.

タンパク質配列:
MSCESSMVLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQWLDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLGKFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAAYLQSDQFCKMPINNKMAQWGNKPVC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
90
Bovine
90
Sheep
90
Dog
90
Rabbit
80
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21266 基板として

Site PTM Type Enzyme
S2 Phosphorylation
T28 Phosphorylation
T30 Phosphorylation
S31 Phosphorylation
K35 Ubiquitination
C39 S-Nitrosylation
R47 Methylation
K54 Sumoylation
K54 Ubiquitination
K56 Ubiquitination
Y66 Phosphorylation
K71 Acetylation
K71 Ubiquitination
K73 Ubiquitination
T75 Phosphorylation
R82 Methylation
K87 Ubiquitination
S122 Phosphorylation
K128 Sumoylation
K128 Ubiquitination
K140 Ubiquitination
S143 Phosphorylation
K177 Ubiquitination
K186 Ubiquitination
K197 Ubiquitination
S204 Phosphorylation
C208 S-Nitrosylation
K209 Ubiquitination
K215 Ubiquitination
K222 Ubiquitination

研究背景

機能:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.

PTMs:

The N-terminus is blocked.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Testis and brain.

サブユニット構造:

Homodimer.

タンパク質ファミリー:

Belongs to the GST superfamily. Mu family.

研究領域

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

参考文献

1). Cardioprotective role of A-cycloglycosylated derivative of Rubiadin in diabetic cardiomyopathy in rats. International Immunopharmacology, 2023 (PubMed: 36989899) [IF=5.6]
2). Propofol inhibits proliferation and migration of glioma cells by up-regulating lncRNA GAS5. TOXICOLOGY IN VITRO, 2022 (PubMed: 35085768) [IF=3.2]

Application: WB    Species: human    Sample: U251 cells

Fig. 5. |Propofol regulates the expression of c-Myc, P21 and GSTM3. (A) After transfected by siRNAs and treated with 8 μg/ml propofol for 6 h, Western blot analysis was conducted to measure effects of propofol on protein expression of c-Myc, P21 and GSTM3 in U251 cells.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.