LETM1 Antibody - #DF12417

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製品: LETM1 Antibody
カタログ: DF12417
タンパク質の説明: Rabbit polyclonal antibody to LETM1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 83 kDa; 83kD(Calculated).
ユニプロット: O95202
RRID: AB_2845222

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(92%), Bovine(92%), Horse(100%), Sheep(92%), Dog(100%), Chicken(92%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
LETM1 Antibody detects endogenous levels of total LETM1.
RRID:
AB_2845222
引用形式: Affinity Biosciences Cat# DF12417, RRID:AB_2845222.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

LETM 1; LETM1 and EF hand domain containing protein 1, mitochondrial; LETM1 and EF-hand domain-containing protein 1; Letm1; LETM1_HUMAN; Leucine zipper EF hand containing transmembrane protein 1; Leucine zipper-EF-hand-containing transmembrane protein 1; Mdm38 homolog; mitochondrial;

免疫原

免疫原:
Uniprot:

O95202(LETM1_HUMAN) >>Visit HPA database.

遺伝子(ID):
LETM1(3954)
タンパク質配列:
MASILLRSCRGRAPARLPPPPRYTVPRGSPGDPAHLSCASTLGLRNCLNVPFGCCTPIHPVYTSSRGDHLGCWALRPECLRIVSRAPWTSTSVGFVAVGPQCLPVRGWHSSRPVRDDSVVEKSLKSLKDKNKKLEEGGPVYSPPAEVVVKKSLGQRVLDELKHYYHGFRLLWIDTKIAARMLWRILNGHSLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSLKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGSATKDFSVFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLIAEEGVDSLNVKELQAACRARGMRALGVTEDRLRGQLKQWLDLHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQKRSEVAKDFEPERVVAAPQRPGTEPQPEMPDTVLQSETLKDTAPVLEGLKEEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYVEESKASKRLTKRVQQMIGQIDGLISQLEMDQQAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDVHISTSQVAEIVATLEKEEKVEEKEKAKEKAEKEVAEVKS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
92
Sheep
92
Xenopus
92
Zebrafish
92
Chicken
92
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95202 基板として

Site PTM Type Enzyme
S3 Phosphorylation
S8 Phosphorylation
S126 Phosphorylation
Y141 Phosphorylation
S142 Phosphorylation
Y164 Phosphorylation
T175 Phosphorylation
S190 Phosphorylation
T192 Phosphorylation
K252 Ubiquitination
K257 Ubiquitination
K284 Acetylation
S287 Phosphorylation
K292 Ubiquitination
R307 Methylation
K373 Ubiquitination
R393 Methylation
Y579 Phosphorylation
S580 Phosphorylation
K597 Acetylation
S602 Phosphorylation
T643 Phosphorylation
S649 Phosphorylation
S666 Phosphorylation
K682 Ubiquitination
S739 Phosphorylation

研究背景

機能:

Mitochondrial proton/calcium antiporter that mediates proton-dependent calcium efflux from mitochondrion. Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization. In contrast to SLC8B1/NCLX, does not constitute the major factor for mitochondrial calcium extrusion.

細胞の位置付け:

Mitochondrion inner membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homohexamer (By similarity). Can form 2 complexes: a major (300 kDa) and a minor complex (500-600 kDa). Interacts with BCS1L.

タンパク質ファミリー:

Belongs to the LETM1 family.

参考文献

1). LETM1 Promotes Gastric Cancer Cell Proliferation, Migration, and Invasion via the PI3K/Akt Signaling Pathway. Journal of Gastric Cancer, 2020 (PubMed: 32595998) [IF=2.5]

Application: WB    Species: human    Sample: GC cell lines and tissues

Fig. 1. |LETM1 is overexpressed in the human GC cell lines and tissues.(D) The protein expression levels of LETM1 in the GC cell lines (MGC-803, AGS, and MKN-45) and GES-1 cells. Data are presented as mean±standard deviation.LETM1 = leucine zipper-EF-hand containing transmembrane protein 1; GC = gastric cancer; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; HR = hazard ratio.*P<0.01.

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