NAT10 Antibody - #DF12427

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製品: NAT10 Antibody
カタログ: DF12427
タンパク質の説明: Rabbit polyclonal antibody to NAT10
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 116 kDa; 116kD(Calculated).
ユニプロット: Q9H0A0
RRID: AB_2845232

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 100ul $280 在庫あり
 200ul $350 在庫あり

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
NAT10 Antibody detects endogenous levels of total NAT10.
RRID:
AB_2845232
引用形式: Affinity Biosciences Cat# DF12427, RRID:AB_2845232.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ALP; DKFZp434C116; FLJ10774; FLJ12179; FLJ23850; hALP; KIAA1709; N acetyltransferase 10; N acetyltransferase 10 GCN5 related; N acetyltransferase like; N acetyltransferase like protein; N-acetyltransferase 10; NAT10; NAT10_HUMAN; NET43;

免疫原

免疫原:
Uniprot:

Q9H0A0(NAT10_HUMAN) >>Visit HPA database.

遺伝子(ID):
NAT10(55226)
タンパク質配列:
MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRKKRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVETVEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLNILPISSHVATMEALPPQTPDESLGPSDLELRELKESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTVALTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLYEVSLQESIRYAPGDAVEKWLNDLLCLDCLNITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKNSPNDLQMLSDAPAHHLFCLLPPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRGKKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIAVHPDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMDMIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H0A0 基板として

Site PTM Type Enzyme
R32 Methylation
K60 Acetylation
K61 Acetylation
K61 Ubiquitination
S67 Phosphorylation
T137 Phosphorylation
S156 Phosphorylation
T164 Phosphorylation
T173 Phosphorylation
K195 Methylation
T223 Phosphorylation
K239 Ubiquitination
K256 Ubiquitination
K262 Ubiquitination
K266 Ubiquitination
S272 Phosphorylation
K274 Ubiquitination
Y416 Phosphorylation
K426 Acetylation
K426 Ubiquitination
S435 Phosphorylation
S438 Phosphorylation
K447 Ubiquitination
K520 Ubiquitination
S589 Phosphorylation
S593 Phosphorylation
K599 Ubiquitination
Y635 Phosphorylation
T666 Phosphorylation
S679 Phosphorylation
T686 Phosphorylation
K689 Ubiquitination
T716 Phosphorylation
T741 Phosphorylation
S809 Phosphorylation
Y820 Phosphorylation
K823 Acetylation
K823 Ubiquitination
K904 Acetylation
K904 Ubiquitination
T929 Phosphorylation
T932 Phosphorylation
S934 Phosphorylation
K942 Acetylation
K942 Ubiquitination
K947 Acetylation
K956 Ubiquitination
S957 Phosphorylation
Y963 Phosphorylation
K978 Ubiquitination
S984 Phosphorylation
S987 Phosphorylation
S990 Phosphorylation

研究背景

機能:

RNA cytidine acetyltransferase that catalyzes the formation of N(4)-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs. Catalyzes ac4C modification of a broad range of mRNAs, enhancing mRNA stability and translation. mRNA ac4C modification is frequently present within wobble cytidine sites and promotes translation efficiency. Mediates the formation of ac4C at position 1842 in 18S rRNA. May also catalyze the formation of ac4C at position 1337 in 18S rRNA (By similarity). Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs (By similarity). Requires the tRNA-binding adapter protein THUMPD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. In addition to RNA acetyltransferase activity, also able to acetylate lysine residues of proteins, such as histones, microtubules, p53/TP53 and MDM2, in vitro. The relevance of the protein lysine acetyltransferase activity is however unsure in vivo. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization.

PTMs:

Acetylation at Lys-426 is required to activation of rRNA transcription. May be autoacetylated; however ability to autoacetylate in vivo requires additional evidences.

細胞の位置付け:

Nucleus>Nucleolus. Midbody.
Note: Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with THUMPD1. Interacts with SUN1 (via N-terminus). Interacts with TERT.

タンパク質ファミリー:

Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.

研究領域

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

参考文献

1). NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma. Acta Pharmaceutica Sinica B, 2022 (PubMed: 35967285) [IF=14.5]

Application: IHC    Species: Human    Sample:

Figure 1 Elevated NAT10 expression is associated with poor outcomes in MM patients. (A) NAT10 mRNA levels were significantly increased in MM samples. The signal level of NAT10 (217884_at signaling) was shown on the y-axis (NP, n = 22; MGUS, n = 44; MM, n = 351). (B) IHC staining verified that NAT10 protein level was elevated in MM patient samples and NAT10 expression was positively correlated with Ki67 expression in MM samples (NP, n = 10; MM, n = 20). (C–F) High expression of NAT10 indicted poor overall survival (OS) in TT2 (C), APEX (D), TT3 (E), and HOVON65 (F) cohorts. All data are displayed as mean ± SD;

Application: WB    Species: Human    Sample:

Figure 1 Elevated NAT10 expression is associated with poor outcomes in MM patients. (A) NAT10 mRNA levels were significantly increased in MM samples. The signal level of NAT10 (217884_at signaling) was shown on the y-axis (NP, n = 22; MGUS, n = 44; MM, n = 351). (B) IHC staining verified that NAT10 protein level was elevated in MM patient samples and NAT10 expression was positively correlated with Ki67 expression in MM samples (NP, n = 10; MM, n = 20). (C–F) High expression of NAT10 indicted poor overall survival (OS) in TT2 (C), APEX (D), TT3 (E), and HOVON65 (F) cohorts. All data are displayed as mean ± SD;

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