NCKAP1 Antibody - #DF12428
| 製品: | NCKAP1 Antibody |
| カタログ: | DF12428 |
| タンパク質の説明: | Rabbit polyclonal antibody to NCKAP1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 115-125 kDa; 129kD(Calculated). |
| ユニプロット: | Q9Y2A7 |
| RRID: | AB_2845233 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12428, RRID:AB_2845233.
折りたたみ／展開
FLJ11291; KIAA0587; Membrane associated protein HEM2; Membrane-associated protein HEM-2; MGC8981; NAP 1; NAP1; NAP125; NCK associated protein 1; NCK associated protein; Nck-associated protein 1; Nckap1; NCKP1_HUMAN; OTTHUMP00000163460; OTTHUMP00000163461; p125Nap1;
免疫原
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
- Q9Y2A7 NCKP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y2A7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| T18 | Phosphorylation | Uniprot | |
| R23 | Methylation | Uniprot | |
| T29 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K201 | Acetylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| K551 | Ubiquitination | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| Y691 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| S838 | Phosphorylation | Uniprot | |
| S840 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| K886 | Ubiquitination | Uniprot | |
| K895 | Ubiquitination | Uniprot | |
| S899 | Phosphorylation | Uniprot | |
| S902 | Phosphorylation | Uniprot | |
| T908 | Phosphorylation | Uniprot | |
| T1045 | Phosphorylation | Uniprot | |
| S1050 | Phosphorylation | Uniprot | |
| S1065 | Phosphorylation | Uniprot | |
| K1075 | Ubiquitination | Uniprot | |
| Y1116 | Phosphorylation | Uniprot | |
| Y1120 | Phosphorylation | Uniprot | |
| S1123 | Phosphorylation | Uniprot |
研究背景
Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.
Cell membrane>Single-pass membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Single-pass membrane protein>Cytoplasmic side.
Note: At the interface between the lamellipodial actin meshwork and the membrane.
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Associates preferentially with the first SH3 domain of NCK. Interacts with NYAP1, NYAP2 and MYO16 (By similarity).
(Microbial infection) Interacts with human cytomegalovirus protein UL135.
Belongs to the HEM-1/HEM-2 family.
研究領域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
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