NFKBIZ Antibody - #DF12429

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製品: NFKBIZ Antibody
カタログ: DF12429
タンパク質の説明: Rabbit polyclonal antibody to NFKBIZ
アプリケーション: WB IHC
Cited expt.:
反応性: Human, Mouse
予測: Bovine, Horse, Sheep, Rabbit
分子量: 64 kDa; 78kD(Calculated).
ユニプロット: Q9BYH8
RRID: AB_2845234

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Bovine(83%), Horse(83%), Sheep(83%), Rabbit(92%)
クローナリティ:
Polyclonal
特異性:
NFKBIZ Antibody detects endogenous levels of total NFKBIZ.
RRID:
AB_2845234
引用形式: Affinity Biosciences Cat# DF12429, RRID:AB_2845234.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ30225; FLJ34463; I-kappa-B-zeta; Ikappa B zeta; IkappaB zeta; IkappaBzeta; IkB-zeta; IKBZ; IKBZ_HUMAN; IKBzeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; MAIL; MAIL protein; Molecule possessing ankyrin repeats induced by lipopolysaccharide; NF-kappa-B inhibitor zeta; NFKBIZ; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor zeta;

免疫原

免疫原:

A synthesized peptide derived from human NFKBIZ, corresponding to a region within the internal amino acids.

Uniprot:

Q9BYH8(IKBZ_HUMAN) >>Visit HPA database.

遺伝子(ID):
NFKBIZ(64332)
発現特異性:
Q9BYH8 IKBZ_HUMAN:

Expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart.

タンパク質配列:
MIVDKLLDDSRGGEGLRDAAGGCGLMTSPLNLSYFYGASPPAAAPGACDASCSVLGPSAPGSPGSDSSDFSSASSVSSCGAVESRSRGGARAERQPVEPHMGVGRQQRGPFQGVRVKNSVKELLLHIRSHKQKASGQAVDDFKTQGVNIEQFRELKNTVSYSGKRKGPDSLSDGPACKRPALLHSQFLTPPQTPTPGESMEDVHLNEPKQESSADLLQNIINIKNECSPVSLNTVQVSWLNPVVVPQSSPAEQCQDFHGGQVFSPPQKCQPFQVRGSQQMIDQASLYQYSPQNQHVEQQPHYTHKPTLEYSPFPIPPQSPAYEPNLFDGPESQFCPNQSLVSLLGDQRESENIANPMQTSSSVQQQNDAHLHSFSMMPSSACEAMVGHEMASDSSNTSLPFSNMGNPMNTTQLGKSLFQWQVEQEESKLANISQDQFLSKDADGDTFLHIAVAQGRRALSYVLARKMNALHMLDIKEHNGQSAFQVAVAANQHLIVQDLVNIGAQVNTTDCWGRTPLHVCAEKGHSQVLQAIQKGAVGSNQFVDLEATNYDGLTPLHCAVIAHNAVVHELQRNQQPHSPEVQELLLKNKSLVDTIKCLIQMGAAVEAKDRKSGRTALHLAAEEANLELIRLFLELPSCLSFVNAKAYNGNTALHVAASLQYRLTQLDAVRLLMRKGADPSTRNLENEQPVHLVPDGPVGEQIRRILKGKSIQQRAPPY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Horse
83
Bovine
83
Sheep
83
Pig
75
Dog
67
Chicken
50
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF-kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. May promote apoptosis (By similarity). Involved in the induction of T helper 17 cells (Th17) differentiation upon recognition of antigen by T cell antigen receptor (TCR) (By similarity).

細胞の位置付け:

Nucleus.
Note: Aggregated in dot-like structures. Colocalizes with NCOR2.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart.

研究領域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

参考文献

1). Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke. Aging and disease, 2023 (PubMed: 37199734) [IF=7.0]

Application: IF/ICC    Species: Mouse    Sample: Mic_M1L1 cells

Figure 4. Subpopulation analysis of M1-polarization-like microglia clusters. (A) Pseudotime plot (from monocle3) shows the differentiation trajectory between Mic_M1L1 and Mic_M1L2 cells. (B) Line plot of the fraction of cells in Mic_M1L1 and Mic_M1L2 subpopulations over time. (C) Expression of the ten genes with the highest Moran’s I score alongside the pseudotime trajectory. (D) Violin plot of the functional pathways revealed by gene set variation analysis (GSVA) in the Mic_M1L1 and Mic_M1L2 subpopulations. (E) Volcano plot displays the differentially expressed genes between Mic_M1L1 and Mic_M1L2 cells. Red dots represent the upregulated genes in the Mic_M1L1 cluster compared with the Mic_M1L2 cluster and blue dots represent the downregulated. (F) Relative activity of the highly expressed transcription factors (TF) in Mic_M1L2 cells compared with Mic_M1L1 cells. (G) Double immunofluorescence staining of Mic_M1L1 cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), Nfkbiz (red, the marker of Mic_M1L1 cells), and DAPI (blue, representing cell nuclei) antibodies (N=3). The yellow bar represents 100μm. The arrows point to the cells simultaneously marked by Iba1 and Nfkbiz. (H) Double immunofluorescence staining of Mic_M1L2 cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), Cd83 (red, the marker of Mic_M1L2 cells), and DAPI (blue, representing cell nuclei) antibodies (N=3). The yellow bar represents 100μm. The arrows point to the cells simultaneously marked by Iba1 and Cd83. (I) Bar plot of the ratio of Nfkbiz+Iba1+ cells (green plus red) compared with all DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6). (J) Bar plot of the ratio of Cd83+Iba1+ cells (green plus red) compared with all DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6).

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