PDHX Antibody - #DF12448
| 製品: | PDHX Antibody |
| カタログ: | DF12448 |
| タンパク質の説明: | Rabbit polyclonal antibody to PDHX |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat, Monkey |
| 予測: | Pig, Bovine, Horse, Sheep, Dog |
| 分子量: | 54 kDa; 54kD(Calculated). |
| ユニプロット: | O00330 |
| RRID: | AB_2845253 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12448, RRID:AB_2845253.
折りたたみ／展開
Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; DLDBP; E3 binding protein; E3-binding protein; E3BP; Lipoyl containing pyruvate dehydrogenase complex component X; Lipoyl-containing pyruvate dehydrogenase complex component X; mitochondrial; ODPX_HUMAN; OPDX; PDHX; PDX 1; PDX1; Pro X; proX; Pyruvate dehydrogenase complex component X; Pyruvate dehydrogenase complex lipoyl containing component X; Pyruvate dehydrogenase complex, E3 binding protein subunit; Pyruvate dehydrogenase protein X component; Pyruvate dehydrogenase protein X component mitochondrial;
免疫原
- O00330 ODPX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATDKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O00330 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y16 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| K120 | Acetylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| T179 | Phosphorylation | Uniprot | |
| K194 | Acetylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| R208 | Methylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| T375 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| K488 | Acetylation | Uniprot | |
| K491 | Acetylation | Uniprot | |
| K491 | Methylation | Uniprot |
研究背景
Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
Delipoylated at Lys-97 by SIRT4, delipoylation decreases the PHD complex activity.
Mitochondrion matrix.
Part of the inner core of the multimeric pyruvate dehydrogenase complex that is composed of about 48 DLAT and 12 PDHX molecules. This core binds multiple copies of pyruvate dehydrogenase (subunits PDH1A and PDHB, E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). Interacts with SIRT4. Interacts with DLD.
Belongs to the 2-oxoacid dehydrogenase family.
研究領域
· Metabolism > Global and overview maps > Metabolic pathways.
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