PDIA6 Antibody - #DF12449
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12449, RRID:AB_2845254.
折りたたみ／展開
Endoplasmic reticulum protein 5; ER protein 5; ERp5; P5; Pdia6; PDIA6_HUMAN; Protein disulfide isomerase A6; Protein disulfide isomerase associated 6; Protein disulfide isomerase family A member 6; Protein disulfide isomerase P5; Protein disulfide isomerase related protein; Protein disulfide-isomerase A6; Thioredoxin domain containing 7 (protein disulfide isomerase); Thioredoxin domain containing protein 7; Thioredoxin domain-containing protein 7; TXNDC7;
免疫原
- Q15084 PDIA6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALLVLGLVSCTFFLAVNGLYSSSDDVIELTPSNFNREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKKDVIELTDDSFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDDLGKDEL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15084 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S22 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K66 | Methylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| K102 | Acetylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| R142 | Methylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| K241 | Acetylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| Y252 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| R392 | Methylation | Uniprot | |
| R409 | Methylation | Uniprot | |
| S428 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
May function as a chaperone that inhibits aggregation of misfolded proteins. Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling. May also regulate the UPR via the EIF2AK3 UPR sensor. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
Endoplasmic reticulum lumen. Cell membrane. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).
Expressed in platelets (at protein level).
Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with MICA on the surface of tumor cells, leading to MICA disulfide bond reduction which is required for its release from tumor cells. Interacts with ITGB3 following platelet stimulation. Interacts with ERN1; the interaction is direct. Interacts with EIF2AK3.
Belongs to the protein disulfide isomerase family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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