PIP5K1A Antibody - #DF12454
製品: | PIP5K1A Antibody |
カタログ: | DF12454 |
タンパク質の説明: | Rabbit polyclonal antibody to PIP5K1A |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Pig, Bovine, Sheep, Rabbit |
分子量: | 62 kDa; 63kD(Calculated). |
ユニプロット: | Q99755 |
RRID: | AB_2845259 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12454, RRID:AB_2845259.
折りたたみ/展開
68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; Phosphatidylinositol 4 phosphate 5 kinase type I alpha; Phosphatidylinositol-4-phosphate 5-kinase type I alpha; Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; PI4P5K Ia; PI51A_HUMAN; PIP5K A; PIP5K1-alpha; Pip5k1a; PIP5KIalpha; PtdIns(4)P-5-kinase 1 alpha;
免疫原
Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
- Q99755 PI51A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASASSGPSSSVGFSSFDPAVPSCTLSSAASGIKRPMASEVLEARQDSYISLVPYASGMPIKKIGHRSVDSSGETTYKKTTSSALKGAIQLGITHTVGSLSTKPERDVLMQDFYVVESIFFPSEGSNLTPAHHYNDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELCSSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPLKPSPSKKFRSGSSFSRRAGSSGNSCITYQPSVSGEHKAQVTTKAEVEPGVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99755 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S48 | Phosphorylation | Uniprot | |
Y55 | Phosphorylation | Uniprot | |
S68 | Phosphorylation | Uniprot | |
S71 | Phosphorylation | Uniprot | |
Y77 | Phosphorylation | Uniprot | |
K78 | Ubiquitination | Uniprot | |
K79 | Ubiquitination | Uniprot | |
K86 | Methylation | Uniprot | |
K103 | Ubiquitination | Uniprot | |
K140 | Ubiquitination | Uniprot | |
Y142 | Phosphorylation | Uniprot | |
K199 | Ubiquitination | Uniprot | |
K206 | Ubiquitination | Uniprot | |
T221 | Phosphorylation | Uniprot | |
K237 | Ubiquitination | Uniprot | |
K261 | Ubiquitination | Uniprot | |
K276 | Ubiquitination | Uniprot | |
T344 | Phosphorylation | Uniprot | |
Y346 | Phosphorylation | Uniprot | |
Y360 | Phosphorylation | Uniprot | |
S366 | Phosphorylation | Uniprot | |
R373 | Methylation | Uniprot | |
K414 | Ubiquitination | Uniprot | |
K452 | Ubiquitination | Uniprot | |
K456 | Ubiquitination | Uniprot | |
S458 | Phosphorylation | Uniprot | |
S460 | Phosphorylation | Uniprot | |
S468 | Phosphorylation | Uniprot | |
S475 | Phosphorylation | Uniprot | |
S476 | Phosphorylation | Uniprot | |
S479 | Phosphorylation | Uniprot | |
T482 | Phosphorylation | Uniprot | |
Y483 | Phosphorylation | Uniprot | |
S486 | Phosphorylation | Uniprot | |
S488 | Phosphorylation | Uniprot | |
K492 | Ubiquitination | Uniprot | |
T516 | Phosphorylation | Uniprot | |
S523 | Phosphorylation | Uniprot | |
S534 | Phosphorylation | Uniprot | |
T540 | Phosphorylation | Uniprot | |
T561 | Phosphorylation | Uniprot |
研究背景
Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes (By similarity).
Cell membrane. Cytoplasm. Nucleus speckle. Cell projection>Ruffle. Cell projection>Lamellipodium. Nucleus.
Note: Colocalizes with RAC1 at actin-rich membrane ruffles. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.
Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
Interacts with RAC1 (By similarity). Interacts with TUT1. Forms a complex with CDH1/E-cadherin, CTNNB1/beta-catenin and CTNND1 at the plasma membrane upon calcium stimulation. Found in a ternary complex with IRS1 and DGKZ in the absence of insulin stimulation (By similarity). Interacts with DGKZ.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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