製品: PIP5K1A Antibody
カタログ: DF12454
タンパク質の説明: Rabbit polyclonal antibody to PIP5K1A
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit
分子量: 62 kDa; 63kD(Calculated).
ユニプロット: Q99755
RRID: AB_2845259

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(90%), Sheep(90%), Rabbit(90%)
クローナリティ:
Polyclonal
特異性:
PIP5K1A Antibody detects endogenous levels of total PIP5K1A.
RRID:
AB_2845259
引用形式: Affinity Biosciences Cat# DF12454, RRID:AB_2845259.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; Phosphatidylinositol 4 phosphate 5 kinase type I alpha; Phosphatidylinositol-4-phosphate 5-kinase type I alpha; Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; PI4P5K Ia; PI51A_HUMAN; PIP5K A; PIP5K1-alpha; Pip5k1a; PIP5KIalpha; PtdIns(4)P-5-kinase 1 alpha;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q99755 PI51A_HUMAN:

Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.

タンパク質配列:
MASASSGPSSSVGFSSFDPAVPSCTLSSAASGIKRPMASEVLEARQDSYISLVPYASGMPIKKIGHRSVDSSGETTYKKTTSSALKGAIQLGITHTVGSLSTKPERDVLMQDFYVVESIFFPSEGSNLTPAHHYNDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELCSSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPLKPSPSKKFRSGSSFSRRAGSSGNSCITYQPSVSGEHKAQVTTKAEVEPGVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
90
Sheep
90
Rabbit
90
Dog
70
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99755 基板として

Site PTM Type Enzyme
S48 Phosphorylation
Y55 Phosphorylation
S68 Phosphorylation
S71 Phosphorylation
Y77 Phosphorylation
K78 Ubiquitination
K79 Ubiquitination
K86 Methylation
K103 Ubiquitination
K140 Ubiquitination
Y142 Phosphorylation
K199 Ubiquitination
K206 Ubiquitination
T221 Phosphorylation
K237 Ubiquitination
K261 Ubiquitination
K276 Ubiquitination
T344 Phosphorylation
Y346 Phosphorylation
Y360 Phosphorylation
S366 Phosphorylation
R373 Methylation
K414 Ubiquitination
K452 Ubiquitination
K456 Ubiquitination
S458 Phosphorylation
S460 Phosphorylation
S468 Phosphorylation
S475 Phosphorylation
S476 Phosphorylation
S479 Phosphorylation
T482 Phosphorylation
Y483 Phosphorylation
S486 Phosphorylation
S488 Phosphorylation
K492 Ubiquitination
T516 Phosphorylation
S523 Phosphorylation
S534 Phosphorylation
T540 Phosphorylation
T561 Phosphorylation

研究背景

機能:

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes (By similarity).

細胞の位置付け:

Cell membrane. Cytoplasm. Nucleus speckle. Cell projection>Ruffle. Cell projection>Lamellipodium. Nucleus.
Note: Colocalizes with RAC1 at actin-rich membrane ruffles. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.

サブユニット構造:

Interacts with RAC1 (By similarity). Interacts with TUT1. Forms a complex with CDH1/E-cadherin, CTNNB1/beta-catenin and CTNND1 at the plasma membrane upon calcium stimulation. Found in a ternary complex with IRS1 and DGKZ in the absence of insulin stimulation (By similarity). Interacts with DGKZ.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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