製品: UBE2E2 Antibody
カタログ: DF12493
タンパク質の説明: Rabbit polyclonal antibody to UBE2E2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Zebrafish, Chicken, Xenopus
分子量: 22 kDa; 22kD(Calculated).
ユニプロット: Q96LR5
RRID: AB_2845298

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(92%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
UBE2E2 Antibody detects endogenous levels of total UBE2E2.
RRID:
AB_2845298
引用形式: Affinity Biosciences Cat# DF12493, RRID:AB_2845298.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

FLJ25157; UB2E2_HUMAN; UBC4/5 homolog yeast; UBCH 8; UbcH8; Ube2e2; Ubiquitin carrier protein E2; Ubiquitin conjugating enzyme E2 E2; Ubiquitin conjugating enzyme E2E 2 (homologous to yeast UBC4/5); Ubiquitin conjugating enzyme E2E 2 (UBC4/5 homolog yeast); Ubiquitin conjugating enzyme E2E 2; Ubiquitin protein ligase E2; Ubiquitin-conjugating enzyme E2 E2; Ubiquitin-protein ligase E2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
100
Chicken
100
Zebrafish
92
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96LR5 基板として

Site PTM Type Enzyme
S2 Acetylation
T3 Phosphorylation
S11 Phosphorylation
S15 Phosphorylation
S18 Phosphorylation
S19 Phosphorylation
K44 Methylation
K48 Acetylation
T49 Phosphorylation
K52 Methylation
S54 Phosphorylation
K62 Ubiquitination
Y85 Phosphorylation
K144 Ubiquitination

研究背景

機能:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.

PTMs:

Autoubiquitinated in vitro.

タンパク質ファミリー:

Belongs to the ubiquitin-conjugating enzyme family.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

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