UFD1L Antibody - #DF12494
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12494, RRID:AB_2845299.
折りたたみ／展開
UB fusion protein 1; Ubiquitin fusion degradation 1 like (yeast); Ubiquitin fusion degradation 1 like; Ubiquitin fusion degradation protein 1 homolog; UFD1; UFD1_HUMAN; UFD1L;
免疫原
Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
- Q92890 UFD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERQVQHEESTEGEADHSGYAGELGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKKVEEDEAGGRFVAFSGEGQSLRKKGRKP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q92890 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| R170 | Methylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| Y261 | Phosphorylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot |
研究背景
Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I.
Nucleus. Cytoplasm>Cytosol.
Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
Heterodimer with NPLOC4, this heterodimer binds VCP and inhibits Golgi membrane fusion. Interacts with USP13. Interacts with ZFAND2B; probably through VCP.
Belongs to the UFD1 family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.