USP33 Antibody - #DF12497
| 製品: | USP33 Antibody |
| カタログ: | DF12497 |
| タンパク質の説明: | Rabbit polyclonal antibody to USP33 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 103 kDa,107 kDa; 107kD(Calculated). |
| ユニプロット: | Q8TEY7 |
| RRID: | AB_2845302 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12497, RRID:AB_2845302.
折りたたみ／展開
Deubiquitinating enzyme 33; EC 3 1 2 15; hVDU1; KIAA1097; MGC16868; OTTHUMP00000011234; OTTHUMP00000011330; OTTHUMP00000011331; pVHL interacting deubiquitinating enzyme 1; Ubiquitin carboxyl terminal hydrolase 33; Ubiquitin carboxyl-terminal hydrolase 33; Ubiquitin specific peptidase 33; Ubiquitin specific processing protease 33; ubiquitin specific protease 33; Ubiquitin thioesterase 33; Ubiquitin thiolesterase 33; Ubiquitin-specific-processing protease 33; UBP33_HUMAN; USP 33; Usp33; VDU 1; VDU1; VHL interacting deubiquitinating enzyme 1; VHL-interacting deubiquitinating enzyme 1;
免疫原
- Q8TEY7 UBP33_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTGSNSHITILTLKVLPHFESLGKQEKIPNKMSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEVEEDPQTITTEETMEEDKSQSDVDFQSCESCSNSDRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTPQILPSNEGVNPRLSASPPKSGNLWPGLAPPHKKAQSASPKRKKQHKKYRSVISDIFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTSIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQNFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGVPPRKAGYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTELEIFIRLNRAFQKEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVMLRQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDILQAEEKIEVETRSL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TEY7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K50 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| T422 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| Y588 | Phosphorylation | Uniprot | |
| K617 | Ubiquitination | Uniprot | |
| T668 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| K714 | Acetylation | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| S736 | Phosphorylation | Uniprot | |
| Y741 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| K877 | Ubiquitination | Uniprot | |
| K882 | Ubiquitination | Uniprot | |
| S907 | Phosphorylation | Uniprot | |
| Y909 | Phosphorylation | Uniprot |
研究背景
Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Associates with centrosomes predominantly in S and G2 phases but less in G1 phase (PubMed:23486064).
Golgi apparatus.
Widely expressed.
Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with ARRB1, ARRB2, ADRB2, DIO2 and ROBO1. Interacts with SELENBP1; in a selenium-dependent manner. Interacts with CCP110.
The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 86 is replaced by a Glu residue.
Belongs to the peptidase C19 family. USP20/USP33 subfamily.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.