Phospho-Atg14 (Ser29) Antibody - #AF2320
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| 製品: | Phospho-Atg14 (Ser29) Antibody |
| カタログ: | AF2320 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Atg14 (Ser29) |
| アプリケーション: | WB IHC |
| Cited expt.: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 65kDa; 55kD(Calculated). |
| ユニプロット: | Q6ZNE5 |
| RRID: | AB_2845334 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF2320, RRID:AB_2845334.
折りたたみ／展開
4832427M01; ATG14; Atg14L; Autophagy-related protein 14-like protein; BAKOR_HUMAN; Barkor; Beclin 1-associated autophagy-related key regulator; D14Ertd114e; D14Ertd436e; KIAA0831; mCG_6911;
免疫原
A synthesized peptide derived from human Atg14 around the phosphorylation site of Ser29.
- Q6ZNE5 BAKOR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism (By similarity).
Cytoplasm. Endoplasmic reticulum membrane>Peripheral membrane protein. Preautophagosomal structure membrane>Peripheral membrane protein. Cytoplasmic vesicle>Autophagosome membrane>Peripheral membrane protein.
Note: Cytosolic under nutrient-rich conditions (PubMed:19050071). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (PubMed:19050071). Accumulates on highly curved PtdIns(3)P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).
The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.
The final 80 residues in the C-terminus define a minimum required region for autophagosome binding called BATS.
The N-terminal cysteine repeats are required for proper localization to the endoplasmic reticulum.
Belongs to the ATG14 family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
参考文献
Application: WB Species: Mouse Sample: brain tissue
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