Phospho-Cytokeratin 20 (Ser13) Antibody - #AF2356
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF2356, RRID:AB_2845370.
折りたたみ／展開
CD20; CK 20; CK-20; CK20; Cytokeratin-20; Cytokeratin20; K1C20_HUMAN; K20; KA20; Keratin 20; keratin 20, type I; keratin 21, rat, homolog of; Keratin; Keratin type I cytoskeletal 20; Keratin-20; Keratin20; KRT 20; KRT 21; KRT20; KRT21; MGC35423; OTTHUMP00000164518; Protein IT; type I cytoskeletal 20;
免疫原
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
- P35900 K1C20_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35900 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | P49137 (MAPKAPK2) , P17252 (PRKCA) | Uniprot |
| S34 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| R42 | Methylation | Uniprot | |
| Y84 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| Y370 | Phosphorylation | Uniprot | |
| Y384 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot |
研究背景
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
Cytoplasm.
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Heterotetramer of two type I and two type II keratins. Associates with KRT8.
Belongs to the intermediate filament family.
研究領域
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.