Phospho-PAK2 (Ser20) Antibody - #AF2381
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF2381, RRID:AB_2845395.
折りたたみ／展開
C-t-PAK2; CB422; EC 2.7.11.1; Gamma PAK; Gamma-PAK; hPAK65; Kinase; p21 (CDKN1A) activated kinase 2; p21 (CDKN1A)-activated kinase 2a; p21 activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein Cdc42 Rac activated kinase 2; p21-activated kinase 2; p21-activated kinase, 65-KD; p21-activated protein kinase I; p21CDKN1A activated kinase 2; p27; p34; p58; p65PAK; PAK 2; PAK-2; PAK-2p34; Pak2; PAK2_HUMAN; PAK65; PAKgamma; S6 H4 kinase; S6/H4 kinase; Serine threonine protein kinase PAK 2; Serine/threonine protein kinase PAK 2;
免疫原
Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
- Q13177 PAK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDNGELEDKPPAPPVRMSSTIFSTGGKDPLSANHSLKPLPSVPEEKKPRHKIISIFSGTEKGSKKKEKERPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSNTVKQKYLSFTPPEKDGFPSGTPALNAKGTEAPAVVTEEEDDDEETAPPVIAPRPDHTKSIYTRSVIDPVPAPVGDSHVDGAAKSLDKQKKKTKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13177 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| S20 | Phosphorylation | P54646 (PRKAA2) , Q13177 (PAK2) | Uniprot |
| T21 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| K38 | Acetylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| S55 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| S58 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K128 | Acetylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| Y130 | Phosphorylation | P12931 (SRC) | Uniprot |
| S132 | Phosphorylation | Uniprot | |
| T134 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| Y139 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| T143 | Phosphorylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S192 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| Y194 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| G213 | Myristoylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| K399 | Acetylation | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| T406 | Phosphorylation | Uniprot | |
| Y443 | Phosphorylation | Uniprot | |
| Y453 | Phosphorylation | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| K492 | Acetylation | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K501 | Acetylation | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot |
PTMs - Q13177 酵素として
| Substrate | Site | Source |
|---|---|---|
| P00519 (ABL1) | S618 | Uniprot |
| P00519 (ABL1) | S619 | Uniprot |
| P01106 (MYC) | T358 | Uniprot |
| P01106 (MYC) | S373 | Uniprot |
| P01106 (MYC) | T400 | Uniprot |
| P01236 (PRL) | S179 | Uniprot |
| P01236 (PRL) | S207 | Uniprot |
| P05412 (JUN) | T2 | Uniprot |
| P05412 (JUN) | T8 | Uniprot |
| P05412 (JUN) | T89 | Uniprot |
| P05412 (JUN) | T93 | Uniprot |
| P05412 (JUN) | T286 | Uniprot |
| P08670 (VIM) | S26 | Uniprot |
| P08670 (VIM) | S39 | Uniprot |
| P08670 (VIM) | S51 | Uniprot |
| P08670 (VIM) | S66 | Uniprot |
| P08670 (VIM) | S73 | Uniprot |
| P17600-2 (SYN1) | S9 | Uniprot |
| P19105 (MYL12A) | S19 | Uniprot |
| P24844 (MYL9) | S20 | Uniprot |
| P31152 (MAPK4) | S186 | Uniprot |
| P35240 (NF2) | S518 | Uniprot |
| P49023 (PXN) | S272 | Uniprot |
| P49023 (PXN) | S274 | Uniprot |
| P55210 (CASP7) | S30 | Uniprot |
| P55210 (CASP7) | T173 | Uniprot |
| P55210 (CASP7) | S239 | Uniprot |
| P62753 (RPS6) | S235 | Uniprot |
| P62753 (RPS6) | S236 | Uniprot |
| P62753 (RPS6) | S240 | Uniprot |
| P62753 (RPS6) | S242 | Uniprot |
| P62805 (HIST4H4) | S48 | Uniprot |
| Q04637 (EIF4G1) | S895 | Uniprot |
| Q05682 (CALD1) | S714 | Uniprot |
| Q05682 (CALD1) | S744 | Uniprot |
| Q13177 (PAK2) | S19 | Uniprot |
| Q13177 (PAK2) | S20 | Uniprot |
| Q13177 (PAK2) | S55 | Uniprot |
| Q13177 (PAK2) | S141 | Uniprot |
| Q13177 (PAK2) | S192 | Uniprot |
| Q13177 (PAK2) | S197 | Uniprot |
| Q13177 (PAK2) | T402 | Uniprot |
| Q15746 (MYLK) | S1208 | Uniprot |
| Q15746 (MYLK) | S1759 | Uniprot |
| Q15746-1 (MYLK) | S1760 | Uniprot |
| Q15796 (SMAD2) | S417 | Uniprot |
| Q16659 (MAPK6) | S189 | Uniprot |
| Q70Z35 (PREX2) | S1107 | Uniprot |
| Q9BUB5 (MKNK1) | S39 | Uniprot |
研究背景
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.
Full-length PAK2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated.
Cytoplasm.
Note: MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.
Nucleus. Cytoplasm>Perinuclear region. Membrane>Lipid-anchor.
Note: Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with SH3MD4. Interacts with SCRIB. Interacts with ARHGEF7 and GIT1. PAK-2p34 interacts with ARHGAP10. Interacts with RAC1.
(Microbial infection) Interacts with and activated by HIV-1 Nef.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
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