製品: Phospho-Semaphorin 4B (Ser825) Antibody
カタログ: AF2400
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Semaphorin 4B (Ser825)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 105kDa; 93kD(Calculated).
ユニプロット: Q9NPR2
RRID: AB_2845414

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-Semaphorin 4B (Ser825) Antibody detects endogenous levels of Semaphorin 4B only when phosphorylated at Ser825.
RRID:
AB_2845414
引用形式: Affinity Biosciences Cat# AF2400, RRID:AB_2845414.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

KIAA1745; MGC131831; Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B; Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain 4B; SEMA4B; SEMAC; Semaphorin 4B; SemC;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MLRTAMGLRSWLAAPWGALPPRPPLLLLLLLLLLLQPPPPTWALSPRISLPLGSEERPFLRFEAEHISNYTALLLSRDGRTLYVGAREALFALSSNLSFLPGGEYQELLWGADAEKKQQCSFKGKDPQRDCQNYIKILLPLSGSHLFTCGTAAFSPMCTYINMENFTLARDEKGNVLLEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSLRPTKTESSLNWLQDPAFVASAYIPESLGSLQGDDDKIYFFFSETGQEFEFFENTIVSRIARICKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLSPSPQDWRDTLFYGVFTSQWHRGTTEGSAVCVFTMKDVQRVFSGLYKEVNRETQQWYTVTHPVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRMLLLQPQARYQRVAVHRVPGLHHTYDVLFLGTGDGRLHKAVSVGPRVHIIEELQIFSSGQPVQNLLLDTHRGLLYAASHSGVVQVPMANCSLYRSCGDCLLARDPYCAWSGSSCKHVSLYQPQLATRPWIQDIEGASAKDLCSASSVVSPSFVPTGEKPCEQVQFQPNTVNTLACPLLSNLATRLWLRNGAPVNASASCHVLPTGDLLLVGTQQLGEFQCWSLEEGFQQLVASYCPEVVEDGVADQTDEGGSVPVIISTSRVSAPAGGKASWGADRSYWKEFLVMCTLFVLAVLLPVLFLLYRHRNSMKVFLKQGECASVHPKTCPVVLPPETRPLNGLGPPSTPLDHRGYQSLSDSPPGSRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPR2 基板として

Site PTM Type Enzyme
N64 N-Glycosylation
Y65 Phosphorylation
T66 Phosphorylation
K403 Acetylation
N405 N-Glycosylation
S694 Phosphorylation
K740 Ubiquitination
K744 Ubiquitination
T764 Phosphorylation
S774 Phosphorylation
T775 Phosphorylation
Y782 Phosphorylation
S784 Phosphorylation
S786 Phosphorylation
S788 Phosphorylation
T796 Phosphorylation
K800 Ubiquitination
S804 Phosphorylation
S808 Phosphorylation
S813 Phosphorylation
S825 Phosphorylation
S830 Phosphorylation

研究背景

機能:

Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

細胞の位置付け:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the semaphorin family.

研究領域

· Organismal Systems > Development > Axon guidance.   (View pathway)

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