Phospho-SRC3 (Thr24) Antibody - #AF2412
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF2412, RRID:AB_2845426.
折りたたみ／展開
ACTR; AIB 1; AIB-1; AIB1; Amplified in breast cancer 1; Amplified in breast cancer 1 protein; bHLHe42; CAGH 16; CAGH16; CBP interacting protein; CBP-interacting protein; Class E basic helix-loop-helix protein 42; CTG 26; CTG26; KAT13B / AIB1; MGC141848; NCOA 3; NCoA-3; Ncoa3; NCOA3_HUMAN; Nuclear receptor coactivator 3; pCIP; RAC 3; RAC-3; RAC3; Receptor associated coactivator 3; Receptor-associated coactivator 3; SRC 3; SRC-3; SRC3; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; TRAM 1; TRAM-1; TRAM1;
免疫原
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
- Q9Y6Q9 NCOA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADVSSTGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYNILHEEDRKDFLKNLPKSTVNGVSWTNETQRQKSHTFNCRMLMKTPHDILEDINASPEMRQRYETMQCFALSQPRAMMEEGEDLQSCMICVARRITTGERTFPSNPESFITRHDLSGKVVNIDTNSLRSSMRPGFEDIIRRCIQRFFSLNDGQSWSQKRHYQEAYLNGHAETPVYRFSLADGTIVTAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGYRPNPNPVGQGIRPPMAGCNSSVGGMSMSPNQGLQMPSSRAYGLADPSTTGQMSGARYGGSSNIASLTPGPGMQSPSSYQNNNYGLNMSSPPHGSPGLAPNQQNIMISPRNRGSPKIASHQFSPVAGVHSPMASSGNTGNHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNITQPSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITAEATGKDTSSITSCGDGNVVKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSDFYNNSISSNGSHLGTKQQVFQGTNSLGLKSSQSVQSIRPPYNRAVSLDSPVSVGSSPPVKNISAFPMLPKQPMLGGNPRMMDSQENYGSSMGGPNRNVTVTQTPSSGDWGLPNSKAGRMEPMNSNSMGRPGGDYNTSLPRPALGGSIPTLPLRSNSIPGARPVLQQQQQMLQMRPGEIPMGMGANPYGQAAASNQLGSWPDGMLSMEQVSHGTQNRPLLRNSLDDLVGPPSNLEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDAFQGQEAAVMMDQKAGLYGQTYPAQGPPMQGGFHLQGQSPSFNSMMNQMNQQGNFPLQGMHPRANIMRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALELKMENPTAGGAAVMRPMMQPQVSSQQGFLNAQMVAQRSRELLSHHFRQQRVAMMMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTQAFSPPPNVTASPSMDGLLAGPTMPQAPPQQFPYQPNYGMGQQPDPAFGRVSSPPNAMMSSRMGPSQNPMMQHPQAASIYQSSEMKGWPSGNLARNSSFSQQQFAHQGNPAVYSMVHMNGSSGHMGQMNMNPMPMSGMPMGPDQKYC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6Q9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| T24 | Phosphorylation | P45983 (MAPK8) , Q16539 (MAPK14) | Uniprot |
| T30 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| K35 | Acetylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| K167 | Ubiquitination | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| R251 | Methylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K276 | Acetylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | P45983 (MAPK8) , Q16539 (MAPK14) | Uniprot |
| S509 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | P45983 (MAPK8) , Q16539 (MAPK14) | Uniprot |
| S551 | Phosphorylation | Uniprot | |
| T557 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| Y574 | Phosphorylation | Uniprot | |
| S601 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
| K616 | Acetylation | Uniprot | |
| K619 | Acetylation | Uniprot | |
| K620 | Acetylation | Uniprot | |
| K620 | Ubiquitination | Uniprot | |
| S636 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| K687 | Acetylation | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| T714 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | Uniprot | |
| K723 | Sumoylation | Uniprot | |
| S728 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K753 | Ubiquitination | Uniprot | |
| K786 | Sumoylation | Uniprot | |
| S794 | Phosphorylation | Uniprot | |
| S836 | Phosphorylation | Uniprot | |
| K840 | Methylation | Uniprot | |
| R849 | Methylation | Uniprot | |
| S857 | Phosphorylation | O15111 (CHUK) , Q16659 (MAPK6) , P17612 (PRKACA) | Uniprot |
| S860 | Phosphorylation | Q16539 (MAPK14) , P45983 (MAPK8) | Uniprot |
| S863 | Phosphorylation | Uniprot | |
| S866 | Phosphorylation | Uniprot | |
| S867 | Phosphorylation | P06493 (CDK1) , Q16539 (MAPK14) , P45983 (MAPK8) | Uniprot |
| K926 | Ubiquitination | Uniprot | |
| R951 | Methylation | Uniprot | |
| S1033 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| S1042 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| S1048 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| T1059 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| S1062 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| T1064 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| T1067 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| K1091 | Methylation | Uniprot | |
| T1114 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| R1171 | Methylation | Uniprot | |
| R1177 | Methylation | Uniprot | |
| R1188 | Methylation | Uniprot | |
| K1194 | Sumoylation | Uniprot | |
| S1330 | Phosphorylation | Uniprot | |
| Y1357 | Phosphorylation | P00519 (ABL1) | Uniprot |
研究背景
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated by CARM1.
Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.
Cytoplasm. Nucleus.
Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
Interacts with CARM1 (By similarity). Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP. Interacts with CASP8AP2, NR3C1 and PCAF. Interacts with ATAD2 and this interaction is enhanced by estradiol. Found in a complex containing NCOA3, AR and MAK. Interacts with DDX5. Interacts with PSMB9. Interacts with NPAS2. Interacts with NR4A3 (By similarity). Interacts with ESRRB; mediates the interaction between ESRRB and RNA polymerase II complexes and allows NCOA3 corecruitment to ESRRB, KLF4, NANOG, and SOX2 enhancer regions to trigger ESRRB-dependent gene activation involved in self-renewal and pluripotency (By similarity).
Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
Belongs to the SRC/p160 nuclear receptor coactivator family.
研究領域
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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