Phospho-MeCP2 (Ser80) Antibody - #AF2435
(1)
(5)
| 製品: | Phospho-MeCP2 (Ser80) Antibody |
| カタログ: | AF2435 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-MeCP2 (Ser80) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 75kDa; 52kD(Calculated). |
| ユニプロット: | P51608 |
| RRID: | AB_2845448 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF2435, RRID:AB_2845448.
折りたたみ／展開
AUTSX 3; AUTSX3; DKFZp686A24160; Mbd 5; Mbd5; MECP 2; MeCP 2 protein; MeCP-2 protein; Mecp2; MECP2_HUMAN; Methyl CpG binding protein 2 (Rett syndrome); Methyl CpG binding protein 2; Methyl-CpG-binding protein 2; MRX 16; MRX 79; MRX16; MRX79; MRXS 13; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP 10; WBP10;
免疫原
- P51608 MECP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51608 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S13 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Q9H2X6 (HIPK2) | Uniprot |
| S86 | Phosphorylation | Uniprot | |
| R115 | Methylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| K119 | Methylation | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| R162 | Methylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K210 | Methylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| K219 | Acetylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| K267 | Methylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K285 | Acetylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| K321 | Acetylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| K347 | Methylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S426 | Phosphorylation | Uniprot | |
| K449 | Acetylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot |
研究背景
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
Nucleus.
Note: Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.
Present in all adult somatic tissues tested.
Interacts with FNBP3 (By similarity). Interacts with CDKL5. Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells (By similarity). Interacts with NCOR2 (By similarity). Interacts with TBL1XR1; bridges interaction between MECP2 and NCOR1. Interacts with TBL1X; recruits TBL1X to the heterochromatin foci.
参考文献
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.