WIPI2 Antibody - #AF2734
| 製品: | WIPI2 Antibody |
| カタログ: | AF2734 |
| タンパク質の説明: | Rabbit polyclonal antibody to WIPI2 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 49kDa; 49kD(Calculated). |
| ユニプロット: | Q9Y4P8 |
| RRID: | AB_2845456 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF2734, RRID:AB_2845456.
折りたたみ／展開
ATG18B; Atg21; CGI 50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain, phosphoinositide interacting 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI 2; WIPI-2; WIPI2; WIPI2_HUMAN; WIPI49 like protein 2; WIPI49-like protein 2;
免疫原
Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.
- Q9Y4P8 WIPI2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLASQSGEAGAGQLLFANFNQDNTEVKGASRAAGLGRRAVVWSLAVGSKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLETVKEKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGAADGYLYMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKGTYVPSSPTRLAYTDDLGAVGGACLEDEASALRLDEDSEHPPMILRTD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y4P8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K28 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K223 | Acetylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| T292 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| T415 | Phosphorylation | Uniprot |
研究背景
Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.
Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella.
Preautophagosomal structure membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Localizes to omegasomes membranes which are endoplasmic reticulum connected structures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.
Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.
Interacts with TECPR1. Interacts with ATG16L1. Interacts with ATG5. Interacts with WIPI1. Interacts with WDR45. May interact with NUDC.
The FRRG motif is required for recruitment to PtdIns3P.
Belongs to the WD repeat SVP1 family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
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