Phospho-HSL (Ser855/Ser554) Antibody - #AF3402
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3402, RRID:AB_2834833.
折りたたみ／展開
Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; Hormone-sensitive lipase; HSL; LHS; Lipase hormone sensitive; LIPE; LIPS_HUMAN;
免疫原
- Q05469 LIPS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPGSKSVSRSDWQPEPHQRPITPLEPGPEKTPIAQPESKTLQGSNTQQKPASNQRPLTQQETPAQHDAESQKEPRAQQKSASQEEFLAPQKPAPQQSPYIQRVLLTQQEAASQQGPGLGKESITQQEPALRQRHVAQPGPGPGEPPPAQQEAESTPAAQAKPGAKREPSAPTESTSQETPEQSDKQTTPVQGAKSKQGSLTELGFLTKLQELSIQRSALEWKALSEWVTDSESESDVGSSSDTDSPATMGGMVAQGVKLGFKGKSGYKVMSGYSGTSPHEKTSARNHRHYQDTASRLIHNMDLRTMTQSLVTLAEDNIAFFSSQGPGETAQRLSGVFAGVREQALGLEPALGRLLGVAHLFDLDPETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQLRALVYYAQRLLVTNRPGVLFFEGDEGLTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTISIGLVSFGEHYKRNETGLSVAASSLFTSGRFAIDPELRGAEFERITQNLDVHFWKAFWNITEMEVLSSLANMASATVRVSRLLSLPPEAFEMPLTADPTLTVTISPPLAHTGPGPVLVRLISYDLREGQDSEELSSLIKSNGQRSLELWPRPQQAPRSRSLIVHFHGGGFVAQTSRSHEPYLKSWAQELGAPIISIDYSLAPEAPFPRALEECFFAYCWAIKHCALLGSTGERICLAGDSAGGNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q05469 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T188 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| S630 | Phosphorylation | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| Y741 | Phosphorylation | Uniprot | |
| T813 | Phosphorylation | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | Uniprot | |
| S853 | Phosphorylation | P17612 (PRKACA) , Q13976 (PRKG1) | Uniprot |
| S855 | Phosphorylation | Q14012 (CAMK1) , P17612 (PRKACA) , Q13131 (PRKAA1) , Q9UQM7 (CAMK2A) | Uniprot |
| K872 | Ubiquitination | Uniprot | |
| S950 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S951 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T955 | Phosphorylation | Uniprot | |
| T1015 | Phosphorylation | Uniprot |
研究背景
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Phosphorylation by AMPK may block translocation to lipid droplets.
Cell membrane. Membrane>Caveola. Cytoplasm>Cytosol.
Note: Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
Interacts with CAVIN1 in the adipocyte cytoplasm. Interacts with PLIN5 (By similarity).
Belongs to the 'GDXG' lipolytic enzyme family.
研究領域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
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