Neutrophil Elastase Antibody - #AF0010

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製品: Neutrophil Elastase Antibody
カタログ: AF0010
タンパク質の説明: Rabbit polyclonal antibody to Neutrophil Elastase
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 29 kDa; 29kD(Calculated).
ユニプロット: P08246
RRID: AB_2845469

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Neutrophil Elastase Antibody detects endogenous levels of total Neutrophil Elastase.
RRID:
AB_2845469
引用形式: Affinity Biosciences Cat# AF0010, RRID:AB_2845469.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Bone marrow serine protease; ELA2; ELANE; Elastase 2; Elastase 2 neutrophil; Elastase neutrophil expressed; Elastase-2; ELNE_HUMAN; GE; Granulocyte derived elastase; HLE; HNE; Human leukocyte elastase; Leukocyte elastase; Medullasin; NE; Neutrophil elastase; PMN E; PMN elastase; Polymorphonuclear elastase; SCN1;

免疫原

免疫原:
Uniprot:

P08246(ELNE_HUMAN) >>Visit HPA database.

遺伝子(ID):
ELANE(1991)
発現特異性:
P08246 ELNE_HUMAN:

Bone marrow cells. Neutrophil (PubMed:10947984).

タンパク質配列:
MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

PTMs - P08246 基板として

Site PTM Type Enzyme
N88 N-Glycosylation
S90 Phosphorylation
N124 N-Glycosylation
C151 S-Nitrosylation
N173 N-Glycosylation

研究背景

機能:

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae.

細胞の位置付け:

Cytoplasmic vesicle>Phagosome.
Note: Localized in phagolysosomes following ingestion of E.coli by neutrophils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Bone marrow cells. Neutrophil.

サブユニット構造:

Interacts with NOTCH2NL.

タンパク質ファミリー:

Belongs to the peptidase S1 family. Elastase subfamily.

研究領域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

参考文献

1). IgD enhances the release of neutrophil extracellular traps (NETs) via FcδR in rheumatoid arthritis patients. International Immunopharmacology, 2023 (PubMed: 36450207) [IF=5.6]
2). Neutrophil extracellular traps promote gastric cancer metastasis by inducing epithelial‑mesenchymal transition. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2021 (PubMed: 34013374) [IF=5.4]

Application: WB    Species: Human    Sample: GC tissues

Figure 3 Abundant NET deposition in human GC tissues. (A) NETs were visualized in GC tissues as extracellular structures decorated with neutrophil elastase (green) and citrullinated H3 (red) co-localizing with DAPI/DNA (blue), but the lack of NETs in normal resection edge. Scale bar, 50 µm; magnification, ×400. (B) Western blot analysis results revealed that the level of cit-H3 was increased in the gastric cancer tissues but nearly absence in normal gastric tissues. The blots are representatives of 3 experiments from 10 pairs of patients with similar results. (C) The values of protein band densities were normalized to cit-h3 protein level of normal group. Data are shown as the means ± SEM, #P<0.001 vs. normal group. NET, neutrophil extracellular trap; GC, gastric cancer; cit-h3, citrullinated histone H3.

Application: IF/ICC    Species: mouse    Sample: tumor

Figure 5.| Effects of NET inhibitors on solid tumor growth in nude mice.(E) Images showing representative immunostaining for neutrophil elastase (green), citrullinated histone H3 (red), and DAPI (blue) in the tumors of mice treated as indicated; scale bar, 50 µm; magnification, x400.
3). Transcriptomics Reveals Effect of Pulsatilla Decoction Butanol Extract in Alleviating Vulvovaginal Candidiasis by Inhibiting Neutrophil Chemotaxis and Activation via TLR4 Signaling. Pharmaceuticals (Basel, Switzerland), 2024 (PubMed: 38794163) [IF=4.6]

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