製品: DSRAD Antibody
カタログ: DF12516
タンパク質の説明: Rabbit polyclonal antibody to DSRAD
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 110 kDa; 136kD(Calculated).
ユニプロット: P55265
RRID: AB_2845478

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(82%)
クローナリティ:
Polyclonal
特異性:
DSRAD Antibody detects endogenous levels of total DSRAD.
RRID:
AB_2845478
引用形式: Affinity Biosciences Cat# DF12516, RRID:AB_2845478.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

136 kDa double-stranded RNA-binding protein; 136kDa double stranded RNA binding protein; Adar 1; ADAR; Adar1; Adenosine deaminase acting on RNA 1 A; Adenosine deaminase RNA specific 1; Adenosine deaminase RNA specific; Adenosine deaminase that act on RNA; AGS6; AV242451; Double stranded RNA specific adenosine deaminase; Double-stranded RNA-specific adenosine deaminase; Double-stranded RNA-specific editase Adar; DRADA; Dsh; Dsrad; DSRAD_HUMAN; dsRNA adenosine deaminase; EC 3.5.4.-; G1P1; IFI 4; IFI-4; IFI4; Ifi4 protein; Interferon induced protein 4; Interferon inducible protein 4; Interferon-inducible protein 4; K88DSRBP; mZaADAR; P136; Pre-mRNA adenosine deaminase; RNA adenosine deaminase 1; RNA-editing deaminase 1; RNA-editing enzyme 1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P55265 DSRAD_HUMAN:

Ubiquitously expressed, highest levels were found in brain and lung (PubMed:7972084). Isoform 5 is expressed at higher levels in astrocytomas as compared to normal brain tissue and expression increases strikingly with the severity of the tumor, being higher in the most aggressive tumors.

タンパク質配列:
MNPRQGYSLSGYYTHPFQGYEHRQLRYQQPGPGSSPSSFLLKQIEFLKGQLPEAPVIGKQTPSLPPSLPGLRPRFPVLLASSTRGRQVDIRGVPRGVHLRSQGLQRGFQHPSPRGRSLPQRGVDCLSSHFQELSIYQDQEQRILKFLEELGEGKATTAHDLSGKLGTPKKEINRVLYSLAKKGKLQKEAGTPPLWKIAVSTQAWNQHSGVVRPDGHSQGAPNSDPSLEPEDRNSTSVSEDLLEPFIAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPEDSNSTSALEDPLEFLDMAEIKEKICDYLFNVSDSSALNLAKNIGLTKARDINAVLIDMERQGDVYRQGTTPPIWHLTDKKRERMQIKRNTNSVPETAPAAIPETKRNAEFLTCNIPTSNASNNMVTTEKVENGQEPVIKLENRQEARPEPARLKPPVHYNGPSKAGYVDFENGQWATDDIPDDLNSIRAAPGEFRAIMEMPSFYSHGLPRCSPYKKLTECQLKNPISGLLEYAQFASQTCEFNMIEQSGPPHEPRFKFQVVINGREFPPAEAGSKKVAKQDAAMKAMTILLEEAKAKDSGKSEESSHYSTEKESEKTAESQTPTPSATSFFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHEPKFQYCVAVGAQTFPSVSAPSKKVAKQMAAEEAMKALHGEATNSMASDNQPEGMISESLDNLESMMPNKVRKIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEAQPKTLPLTGSTFHDQIAMLSHRCFNTLTNSFQPSLLGRKILAAIIMKKDSEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFKKGLKDMGYGNWISKPQEEKNFYLCPV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
82
Zebrafish
82
Chicken
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55265 基板として

Site PTM Type Enzyme
R4 Methylation
S10 Phosphorylation
T14 Phosphorylation
R26 Methylation
K48 Methylation
K48 Ubiquitination
R91 Methylation
R95 Methylation
S112 Phosphorylation
R114 Methylation
Y136 Phosphorylation
K154 Ubiquitination
K164 Ubiquitination
T167 Phosphorylation
Y177 Phosphorylation
K181 Ubiquitination
K187 Ubiquitination
T191 Phosphorylation
K302 Ubiquitination
K320 Ubiquitination
K326 Ubiquitination
T349 Phosphorylation
K358 Ubiquitination
K366 Ubiquitination
S371 Phosphorylation
K384 Sumoylation
K384 Ubiquitination
K418 Sumoylation
K418 Ubiquitination
K433 Ubiquitination
Y438 Phosphorylation
S442 Phosphorylation
Y446 Phosphorylation
S465 Phosphorylation
S481 Phosphorylation
Y483 Phosphorylation
S484 Phosphorylation
S491 Phosphorylation
K495 Ubiquitination
S553 Phosphorylation
K558 Sumoylation
K558 Ubiquitination
K564 Ubiquitination
T567 Phosphorylation
K574 Acetylation
K574 Sumoylation
K574 Ubiquitination
K576 Ubiquitination
S578 Phosphorylation
K580 Sumoylation
K580 Ubiquitination
Y587 Phosphorylation
S588 Phosphorylation
K591 Ubiquitination
K595 Ubiquitination
T596 Phosphorylation
S599 Phosphorylation
T601 Phosphorylation
T603 Phosphorylation
S605 Phosphorylation
T607 Phosphorylation
S608 Phosphorylation
S611 Phosphorylation
K613 Ubiquitination
S614 Phosphorylation
T617 Phosphorylation
T618 Phosphorylation
K625 Ubiquitination
S629 Phosphorylation
S636 Phosphorylation
K637 Ubiquitination
Y648 Phosphorylation
K665 Acetylation
K665 Ubiquitination
K666 Acetylation
K666 Ubiquitination
K669 Sumoylation
K669 Ubiquitination
S701 Phosphorylation
S707 Phosphorylation
K712 Ubiquitination
K715 Ubiquitination
Y722 Phosphorylation
Y734 Phosphorylation
K745 Ubiquitination
K757 Acetylation
K757 Ubiquitination
K763 Ubiquitination
K781 Ubiquitination
K798 Acetylation
K798 Ubiquitination
T808 Phosphorylation
T811 Phosphorylation
S814 Phosphorylation
T818 Phosphorylation
S823 Phosphorylation
S825 Phosphorylation
K831 Ubiquitination
K895 Ubiquitination
K902 Acetylation
K936 Ubiquitination
K944 Ubiquitination
K948 Ubiquitination
S975 Phosphorylation
S986 Phosphorylation
K996 Ubiquitination
K1003 Sumoylation
K1003 Ubiquitination
S1089 Phosphorylation
R1096 Methylation
K1105 Acetylation
S1110 Phosphorylation
S1114 Phosphorylation
K1115 Acetylation
K1115 Ubiquitination
K1159 Acetylation
K1166 Acetylation
K1196 Ubiquitination
K1214 Ubiquitination
K1219 Ubiquitination

研究背景

機能:

Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assembly of viral particles. However, high levels of ADAR1 inhibit HDV replication.

PTMs:

Sumoylation reduces RNA-editing activity.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Shuttles between the cytoplasm and nucleus (PubMed:7565688, PubMed:24753571). Nuclear import is mediated by TNPO1 (PubMed:24753571).

Cytoplasm. Nucleus. Nucleus>Nucleolus.
Note: Predominantly nuclear but can shuttle between nucleus and cytoplasm. TNPO1 can mediate its nuclear import whereas XPO5 can mediate its nuclear export.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed, highest levels were found in brain and lung. Isoform 5 is expressed at higher levels in astrocytomas as compared to normal brain tissue and expression increases strikingly with the severity of the tumor, being higher in the most aggressive tumors.

サブユニット構造:

Homodimer. Homodimerization is essential for its catalytic activity. Isoform 5 can form heterodimers with ADARB1/ADAR2. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90. Binding to ILF3/NF90 up-regulates ILF3-mediated gene expression. Isoform 1 and isoform 5 (via DRBM 3 domain) interact with TNPO1. Isoform 5 (via DRBM domains) interacts with XPO5. Isoform 1 and isoform 5 can interact with EIF2AK2/PKR and UPF1.

タンパク質ファミリー:

The first DRADA repeat binds Z-DNA.

The third dsRNA-binding domain (DRBM 3) contains an additional N-terminal alpha-helix that is part of a bi-partite nuclear localization signal, together with the sequence immediately C-terminal to DRBM 3. The presence of DRBM 3 is important to bring together the N-terminal and the C-terminal part of the bi-partite nuclear localization signal for import mediated by TNPO1 (PubMed:24753571). RNA binding interferes with nuclear import (PubMed:19124606, PubMed:24753571).

研究領域

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

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