M-CSF Antibody - #DF12536
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| 製品: | M-CSF Antibody |
| カタログ: | DF12536 |
| タンパク質の説明: | Rabbit polyclonal antibody to M-CSF |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Sheep, Rabbit |
| 分子量: | 70 kDa; 60kD(Calculated). |
| ユニプロット: | P09603 |
| RRID: | AB_2845498 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12536, RRID:AB_2845498.
折りたたみ／展開
Colony stimulating factor 1 (macrophage); Colony stimulating factor 1; Colony stimulating factor macrophage specific; CSF 1; CSF-1; CSF1; CSF1_HUMAN; Csfm; Lanimostim; M CSF; M-CSF; Macrophage Colony Stimulating Factor 1; Macrophage colony stimulating factor; MCSF; MGC31930; Processed macrophage colony-stimulating factor 1;
免疫原
- P09603 CSF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTAPGAAGRCPPTTWLGSLLLLVCLLASRSITEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCNCLYPKAIPSSDPASVSPHQPLAPSMAPVAGLTWEDSEGTEGSSLLPGEQPLHTVDPGSAKQRPPRSTCQSFEPPETPVVKDSTIGGSPQPRPSVGAFNPGMEDILDSAMGTNWVPEEASGEASEIPVPQGTELSPSRPGGGSMQTEPARPSNFLSASSPLPASAKGQQPADVTGTALPRVGPVRPTGQDWNHTPQKTDHPSALLRDPPEPGSPRISSLRPQGLSNPSTLSAQPQLSRSHSSGSVLPLGELEGRRSTRDRRSPAEPEGGPASEGAARPLPRFNSVPLTDTGHERQSEGSFSPQLQESVFHLLVPSVILVLLAVGGLLFYRWRRRSHQEPQRADSPLEQPEGSPLTQDDRQVELPV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P09603 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S256 | O-Glycosylation | Uniprot | |
| T257 | O-Glycosylation | Uniprot | |
| T266 | O-Glycosylation | Uniprot | |
| T266 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| T363 | O-Glycosylation | Uniprot | |
| T365 | O-Glycosylation | Uniprot | |
| T376 | O-Glycosylation | Uniprot | |
| T383 | O-Glycosylation | Uniprot | |
| T387 | O-Glycosylation | Uniprot | |
| S391 | O-Glycosylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| S426 | Phosphorylation | Uniprot | |
| S428 | O-Glycosylation | Uniprot | |
| S430 | O-Glycosylation | Uniprot | |
| S431 | O-Glycosylation | Uniprot | |
| S433 | O-Glycosylation | Uniprot | |
| S451 | O-Glycosylation | Uniprot | |
| S461 | O-Glycosylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S473 | O-Glycosylation | Uniprot | |
| T477 | O-Glycosylation | Uniprot | |
| S533 | Phosphorylation | Uniprot |
研究背景
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated.
Cell membrane>Single-pass type I membrane protein.
Secreted>Extracellular space.
Homodimer or heterodimer; disulfide-linked. Interacts with CSF1R.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
参考文献
Application: WB Species: Human Sample: SaOS-2 cells
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