NUCL Antibody - #DF12542

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製品: NUCL Antibody
カタログ: DF12542
タンパク質の説明: Rabbit polyclonal antibody to NUCL
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Sheep, Rabbit, Dog, Xenopus
分子量: 110 kDa; 77kD(Calculated).
ユニプロット: P19338
RRID: AB_2845504

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Sheep(100%), Rabbit(83%), Dog(100%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
NUCL Antibody detects endogenous levels of total NUCL.
RRID:
AB_2845504
引用形式: Affinity Biosciences Cat# DF12542, RRID:AB_2845504.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C23; FLJ45706; MS1116; NCL; Nucl; NUCL_HUMAN; Nucleolin; Protein C23;

免疫原

免疫原:

A synthesized peptide derived from human NUCL, corresponding to a region within the internal amino acids.

Uniprot:

P19338(NUCL_HUMAN) >>Visit HPA database.

遺伝子(ID):
NCL(4691)
タンパク質配列:
MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Xenopus
88
Rabbit
83
Chicken
75
Horse
0
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

PTMs:

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).

Symmetrically methylated by PRMT5.

細胞の位置付け:

Nucleus>Nucleolus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

研究領域

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

参考文献

1). A novel lncRNA SNHG29 regulates EP300- related histone acetylation modification and inhibits FLT3-ITD AML development. Leukemia, 2023 (PubMed: 37157016) [IF=12.8]
2). Nucleolin Malonylation as a Nuclear-Cytosol Signal Exchange Mechanism to Drive Cell Proliferation in Hepatocarcinoma by Enhancing AKT Translation. The Journal of biological chemistry, 2024 (PubMed: 39305961) [IF=4.0]
3). Dimethyl itaconate inhibits LPS‑induced microglia inflammation and inflammasome‑mediated pyroptosis via inducing autophagy and regulating the Nrf‑2/HO‑1 signaling pathway. Molecular Medicine Reports, 2021 (PubMed: 34296312) [IF=3.4]

Application: WB    Species: Mice    Sample: microglial cells

Figure 5. DI regulates microglia inflammatory profile via inhibiting NF-κB phosphorylation and nuclear translocation. Levels of cytokines, including (A) iNOS, (B) TNF-α, (C) IL-6, (D) IFN-γ, (E) IL-18 and (F) IL-10, were determined by ELISA. (G) Nuclear translocation of p-NF-κB was determined by immunofluorescence staining. Scale bar, 100 µm. (H and I) p-NF-κB expression in the nucleus were determined by western blotting. (J and K) Ratio of whole cell p-NF-κB/total NF-κB expression was determined by western blotting. Data are presented as the mean ± standard error of the mean (n=3). **P<0.01, ***P<0.001 vs. sham group; #P<0.05, ##P<0.01, ###P<0.001 vs. LPS+ATP group. DI, dimethyl itaconate; iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; p-, phosphorylated.

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