CCL8 Antibody - #DF12545

(1)   (2)  
製品: CCL8 Antibody
カタログ: DF12545
タンパク質の説明: Rabbit polyclonal antibody to CCL8
アプリケーション: WB
反応性: Human
予測: Sheep
分子量: 11 kDa; 11kD(Calculated).
ユニプロット: P80075
RRID: AB_2845507

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Sheep(89%)
クローナリティ:
Polyclonal
特異性:
CCL8 Antibody detects endogenous levels of total CCL8.
RRID:
AB_2845507
引用形式: Affinity Biosciences Cat# DF12545, RRID:AB_2845507.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Ccl8; CCL8_HUMAN; HC14; MCP-2; MCP-2(6-76); Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; SCYA10; SCYA8; Small-inducible cytokine A8;

免疫原

免疫原:
Uniprot:

P80075(CCL8_HUMAN) >>Visit HPA database.

遺伝子(ID):
CCL8(6355)
発現特異性:
P80075 CCL8_HUMAN:

Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.

タンパク質配列:
MKVSAALLCLLLMAATFSPQGLAQPDSVSIPITCCFNVINRKIPIQRLESYTRITNIQCPKEAVIFKTKRGKEVCADPKERWVRDSMKHLDQIFQNLKP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
89
Pig
78
Bovine
67
Dog
67
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.

PTMs:

N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.

サブユニット構造:

Monomer or homodimer; in equilibrium.

タンパク質ファミリー:

Belongs to the intercrine beta (chemokine CC) family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

参考文献

1). C-C Motif Chemokine 8 promotes angiogenesis in vascular endothelial cells. Vascular, 2021 (PubMed: 32972333) [IF=1.1]

Application: WB    Species: human    Sample: MA and SA

Figure 1. |Expression of CCL8 in human carotid plaques with mild angiogenesis (MA) and severe angiogenesis (SA). (c) CCL8 protein expression in in SA group was higher than MA group.CCL8: C-C Motif Chemokine 8.

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