SIKE1 Antibody - #DF12548
| 製品: | SIKE1 Antibody |
| カタログ: | DF12548 |
| タンパク質の説明: | Rabbit polyclonal antibody to SIKE1 |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 24 kDa; 24kD(Calculated). |
| ユニプロット: | Q9BRV8 |
| RRID: | AB_2845510 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12548, RRID:AB_2845510.
折りたたみ／展開
FLJ21168; RGD1311316; RP5-1000E10.4; SIKE; sike1; SIKE1_HUMAN; Suppressor of IKBKE 1; Suppressor of IKK-epsilon;
免疫原
Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level).
- Q9BRV8 SIKE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSCTIEKILTDAKTLLERLREHDAAAESLVDQSAALHRRVAAMREAGTALPDQYQEDASDMKDMSKYKPHILLSQENTQIRDLQQENRELWISLEEHQDALELIMSKYRKQMLQLMVAKKAVDAEPVLKAHQSHSAEIESQIDRICEMGEVMRKAVQVDDDQFCKIQEKLAQLELENKELRELLSISSESLQARKENSMDTASQAIK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BRV8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K13 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Q9UHD2 (TBK1) | Uniprot |
| K154 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Q9UHD2 (TBK1) | Uniprot |
| S187 | Phosphorylation | Q9UHD2 (TBK1) | Uniprot |
| S188 | Phosphorylation | Q9UHD2 (TBK1) | Uniprot |
| S190 | Phosphorylation | Q9UHD2 (TBK1) | Uniprot |
| K195 | Ubiquitination | Uniprot | |
| S198 | Phosphorylation | Q9UHD2 (TBK1) | Uniprot |
研究背景
Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.
Cytoplasm.
Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level).
Interacts with IKBKE and TBK1 via its coiled coil region. Interaction with TBK1 is disrupted upon viral infection or TLR3 stimulation. Interacts with CDC42BPB.
Belongs to the SIKE family.
研究領域
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
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