AMOTL1 Antibody - #DF12556
(1)
| 製品: | AMOTL1 Antibody |
| カタログ: | DF12556 |
| タンパク質の説明: | Rabbit polyclonal antibody to AMOTL1 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Horse, Rabbit, Dog |
| 分子量: | 107 kDa; 107kD(Calculated). |
| ユニプロット: | Q8IY63 |
| RRID: | AB_2845518 |
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(82%), Horse(91%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
AMOTL1 Antibody detects endogenous levels of total AMOTL1.
RRID:
AB_2845518
引用形式: Affinity Biosciences Cat# DF12556, RRID:AB_2845518.
引用形式: Affinity Biosciences Cat# DF12556, RRID:AB_2845518.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
2310010G08Rik; 2310067L22Rik; 4932416D09Rik; AMOL1_HUMAN; Amotl1; Angiomotin-like protein 1; FLJ00155; JEAP; Junction enriched and associated protein; mFLJ00155;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
- Q8IY63 AMOL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWRAKLRRGTCEPAVKGSPSACYSPSSPVQVLEDSTYFSPDFQLYSGRHETSALTVEATSSIREKVVEDPLCNFHSPNFLRISEVEMRGSEDAAAGTVLQRLIQEQLRYGTPTENMNLLAIQHQATGSAGPAHPTNNFSSTENLTQEDPQMVYQSARQEPQGQEHQVDNTVMEKQVRSTQPQQNNEELPTYEEAKAQSQFFRGQQQQQQQQGAVGHGYYMAGGTSQKSRTEGRPTVNRANSGQAHKDEALKELKQGHVRSLSERIMQLSLERNGAKQHLPGSGNGKGFKVGGGPSPAQPAGKVLDPRGPPPEYPFKTKQMMSPVSKTQEHGLFYGDQHPGMLHEMVKPYPAPQPVRTDVAVLRYQPPPEYGVTSRPCQLPFPSTMQQHSPMSSQTSSASGPLHSVSLPLPLPMALGAPQPPPAASPSQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGYYDNADKLHKFEKELQRISEAYESLVKSTTKRESLDKAMRNKLEGEIRRLHDFNRDLRDRLETANRQLSSREYEGHEDKAAEGHYASQNKEFLKEKEKLEMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREKQAYVEKVEKLQQALTQLQSACEKREQMERRLRTWLERELDALRTQQKHGNGQPANMPEYNAPALLELVREKEERILALEADMTKWEQKYLEESTIRHFAMNAAATAAAERDTTIINHSRNGSYGESSLEAHIWQEEEEVVQANRRCQDMEYTIKNLHAKIIEKDAMIKVLQQRSRKDAGKTDSSSLRPARSVPSIAAATGTHSRQTSLTSSQLAEEKKEEKTWKGSIGLLLGKEHHEHASAPLLPPPPTSALSSIASTTAASSAHAKTGSKDSSTQTDKSAELFWPSMASLPSRGRLSTTPAHSPVLKHPAAKGTAEKLENSPGHGKSPDHRGRVSSLLHKPEFPDGEMMEVLI
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Dog
100
Horse
91
Pig
82
Bovine
73
Sheep
73
Chicken
73
Xenopus
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IY63 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K16 | Ubiquitination | Uniprot | |
| T51 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| Y191 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| Y218 | Phosphorylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K316 | Methylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K318 | Methylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| Y546 | Phosphorylation | Uniprot | |
| K551 | Ubiquitination | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| Y661 | Phosphorylation | Uniprot | |
| K686 | Ubiquitination | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| T707 | Phosphorylation | Uniprot | |
| T714 | Phosphorylation | Uniprot | |
| T715 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K761 | Ubiquitination | Uniprot | |
| K765 | Ubiquitination | Uniprot | |
| K782 | Ubiquitination | Uniprot | |
| S793 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S796 | Phosphorylation | Uniprot | |
| T803 | Phosphorylation | Uniprot | |
| S805 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S813 | Phosphorylation | Uniprot | |
| S828 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| T877 | Phosphorylation | Uniprot | |
| T879 | Phosphorylation | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| S895 | Phosphorylation | Uniprot | |
| S900 | Phosphorylation | Uniprot | |
| T901 | Phosphorylation | Uniprot | |
| T902 | Phosphorylation | Uniprot | |
| S906 | Phosphorylation | Uniprot | |
| T917 | Phosphorylation | Uniprot | |
| S924 | Phosphorylation | Uniprot | |
| S930 | Phosphorylation | Uniprot | |
| S939 | Phosphorylation | Uniprot |
研究背景
機能:
Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus.
PTMs:
Polyubiquitinated by NEDD4, leading to proteasomal degradation.
細胞の位置付け:
Cell junction>Tight junction.
タンパク質ファミリー:
Belongs to the angiomotin family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.