製品: ANGPTL2 Antibody
カタログ: DF12557
タンパク質の説明: Rabbit polyclonal antibody to ANGPTL2
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 57 kDa; 57kD(Calculated).
ユニプロット: Q9UKU9
RRID: AB_2845519

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)
クローナリティ:
Polyclonal
特異性:
ANGPTL2 Antibody detects endogenous levels of total ANGPTL2.
RRID:
AB_2845519
引用形式: Affinity Biosciences Cat# DF12557, RRID:AB_2845519.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

AI593246; Angiopoietin like 2; Angiopoietin related protein 2; Angiopoietin-like protein 2; Angiopoietin-related protein 2; ANGL2_HUMAN; Angptl2; Arp2; AW260363; HARP; MGC8889; UNQ170/PRO196;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9UKU9 ANGL2_HUMAN:

Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.

タンパク質配列:
MRPLCVTCWWLGLLAAMGAVAGQEDGFEGTEEGSPREFIYLNRYKRAGESQDKCTYTFIVPQQRVTGAICVNSKEPEVLLENRVHKQELELLNNELLKQKRQIETLQQLVEVDGGIVSEVKLLRKESRNMNSRVTQLYMQLLHEIIRKRDNALELSQLENRILNQTADMLQLASKYKDLEHKYQHLATLAHNQSEIIAQLEEHCQRVPSARPVPQPPPAAPPRVYQPPTYNRIINQISTNEIQSDQNLKVLPPPLPTMPTLTSLPSSTDKPSGPWRDCLQALEDGHDTSSIYLVKPENTNRLMQVWCDQRHDPGGWTVIQRRLDGSVNFFRNWETYKQGFGNIDGEYWLGLENIYWLTNQGNYKLLVTMEDWSGRKVFAEYASFRLEPESEYYKLRLGRYHGNAGDSFTWHNGKQFTTLDRDHDVYTGNCAHYQKGGWWYNACAHSNLNGVWYRGGHYRSRYQDGVYWAEFRGGSYSLKKVVMMIRPNPNTFH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
89
Chicken
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKU9 基板として

Site PTM Type Enzyme
S73 Phosphorylation
K86 Methylation
T229 O-Glycosylation
Y426 Phosphorylation
Y433 Phosphorylation
Y453 Phosphorylation
Y462 Phosphorylation
Y467 Phosphorylation
Y476 Phosphorylation

研究背景

機能:

Induces sprouting in endothelial cells through an autocrine and paracrine action.

PTMs:

N-glycosylated.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.

参考文献

1). Anti-inflammatory activity of berberine in non-alcoholic fatty liver disease via the Angptl2 pathway. BMC IMMUNOLOGY, 2020 (PubMed: 32429849) [IF=3.0]

Application: WB    Species: rat    Sample: liver

Fig. 5 |The protein levels of Angptl2, Foxo1, NF-κB, p-NF-κB and CCR2 were measured by Western blot analysis and normalized by GAPDH expression. Data are presented as mean ± SD from eight rats. **P < 0.01,* P < 0.05.n = 8/group

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For Research Use Only.
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