CHMP2B Antibody - #DF12582
製品: | CHMP2B Antibody |
カタログ: | DF12582 |
タンパク質の説明: | Rabbit polyclonal antibody to CHMP2B |
アプリケーション: | WB |
反応性: | Human, Mouse, Rat |
予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 32 kDa; 24kD(Calculated). |
ユニプロット: | Q9UQN3 |
RRID: | AB_2845544 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12582, RRID:AB_2845544.
折りたたみ/展開
ALS17; Charged multivesicular body protein 2b; CHM2B_HUMAN; CHMP family, member 2B; CHMP2.5; CHMP2b; Chromatin modifying protein 2b; Chromatin-modifying protein 2b; DMT1; hVps2-2; Vacuolar protein sorting 2, yeast, homolog of, B; Vacuolar protein sorting 2-2; Vacuolar protein sorting-associated protein 2-2; VPS2 homolog B; Vps2-2; VPS2B;
免疫原
Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
- Q9UQN3 CHM2B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASLFKKKTVDDVIKEQNRELRGTQRAIIRDRAALEKQEKQLELEIKKMAKIGNKEACKVLAKQLVHLRKQKTRTFAVSSKVTSMSTQTKVMNSQMKMAGAMSTTAKTMQAVNKKMDPQKTLQTMQNFQKENMKMEMTEEMINDTLDDIFDGSDDEEESQDIVNQVLDEIGIEISGKMAKAPSAARSLPSASTSKATISDEEIERQLKALGVD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UQN3 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
K6 | Methylation | Uniprot | |
K7 | Methylation | Uniprot | |
T9 | Phosphorylation | Uniprot | |
K15 | Ubiquitination | Uniprot | |
R22 | Methylation | Uniprot | |
T24 | Phosphorylation | Uniprot | |
K63 | Ubiquitination | Uniprot | |
S79 | Phosphorylation | Uniprot | |
K81 | Ubiquitination | Uniprot | |
S86 | Phosphorylation | Uniprot | |
T89 | Phosphorylation | Uniprot | |
K90 | Ubiquitination | Uniprot | |
S103 | Phosphorylation | Uniprot | |
K107 | Ubiquitination | Uniprot | |
K114 | Acetylation | Uniprot | |
K120 | Ubiquitination | Uniprot | |
K130 | Ubiquitination | Uniprot | |
S153 | Phosphorylation | Uniprot | |
S187 | Phosphorylation | Uniprot | |
S190 | Phosphorylation | Uniprot | |
S194 | Phosphorylation | Uniprot | |
K195 | Ubiquitination | Uniprot | |
T197 | Phosphorylation | Uniprot | |
S199 | Phosphorylation | Uniprot |
研究背景
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
Cytoplasm>Cytosol. Late endosome membrane>Peripheral membrane protein.
Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. Interacts with CHMP2A. Interacts with VPS4A. Interacts with VPS4B; the interaction is direct.
The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components (By similarity).
Belongs to the SNF7 family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
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