Cytohesin2 Antibody - #DF12592
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| 製品: | Cytohesin2 Antibody |
| カタログ: | DF12592 |
| タンパク質の説明: | Rabbit polyclonal antibody to Cytohesin2 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Dog |
| 分子量: | 47 kDa; 47kD(Calculated). |
| ユニプロット: | Q99418 |
| RRID: | AB_2845554 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12592, RRID:AB_2845554.
折りたたみ／展開
ARF exchange factor; ARF nucleotide binding site opener; ARF nucleotide-binding site opener; Arno; ARNO protein; CLM2; CTS18; CTS18.1; CYH2_HUMAN; Cyth2; Cytohesin 2; Cytohesin-2; MGC137537; MGC80440; PH; PH, SEC7 and coiled-coil domain-containing protein 2; Pleckstrin homology Sec7 and coiled coil domains 2; Pleckstrin homology Sec7 and coiled coil domains protein 2; Protein ARNO; PSCD2; PSCD2L; PSCD2L, formerly; SEC7 and coiled-coil domain-containing protein 2; Sec7; SEC7 homolog B; Sec7B; SEC7L; Sec7p L; Sec7p-like; Sec7pL;
免疫原
- Q99418 CYH2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99418 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K26 | Ubiquitination | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| T58 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| Y235 | Phosphorylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| T276 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K319 | Ubiquitination | Uniprot | |
| T360 | Phosphorylation | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| Y381 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | P05771 (PRKCB) , P05129 (PRKCG) , P17252 (PRKCA) | Uniprot |
研究背景
Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP (By similarity). The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Involved in neurite growth (By similarity).
Cell membrane>Peripheral membrane protein. Cytoplasm. Cell projection. Cell projection>Growth cone. Cell junction>Tight junction. Cell junction>Adherens junction.
Note: Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. They require also interaction with phosphoinositides for targeting to plasma membrane (PubMed:17398095). In differentiating neuroblastoma cells, colocalizes with CCDC120 in both neurite shaft and growth cone areas.
Widely expressed.
Heteromer. Composed of GRASP, CYTH2 and at least one GRM1 (By similarity). Interacts with ARRB1. Interacts with ARL4D; the interaction is direct. Directly interacts with CCDC120 through the coiled coil domain; this interaction stabilizes CCDC120, possibly by preventing its ubiquitination, and is required for neurite growth in neuroblastoma cells. Interacts with ARF1. Interacts with FRMD4A (By similarity). Interacts (via N-terminal domain) with INAVA (via N-terminal domain).
Binds via its PH domain to the inositol head group of phosphatidylinositol 1,4,5-trisphosphate. The PH domain is necessary and sufficient for plasma membrane relocalization.
Autoinhibited by its C-terminal basic region.
The coiled coil domain is involved in interaction with CCDC120.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
参考文献
Application: WB Species: human Sample: MHCC97H cells
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