DBNL Antibody - #DF12594
| 製品: | DBNL Antibody |
| カタログ: | DF12594 |
| タンパク質の説明: | Rabbit polyclonal antibody to DBNL |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 55 kDa; 48kD(Calculated). |
| ユニプロット: | Q9UJU6 |
| RRID: | AB_2845556 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12594, RRID:AB_2845556.
折りたたみ／展開
ABP1; Actin-binding protein 1; Cervical mucin associated protein; Cervical mucin-associated protein; Cervical SH3P7; CMAP; Dbnl; DBNL_HUMAN; Drebrin F; drebrin like; Drebrin like protein; Drebrin-F; Drebrin-like protein; HIP 55; HIP-55; HPK1 interacting protein of 55 kDa; HPK1-interacting protein of 55 kDa; SH3 domain containing protein 7; SH3 domain-containing protein 7; SH3P7; src homology 3 domain containing protein HIP 55;
免疫原
- Q9UJU6 DBNL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAANLSRNGPALQEAYVRVVTEKSPTDWALFTYEGNSNDIRVAGTGEGGLEEMVEELNSGKVMYAFCRVKDPNSGLPKFVLINWTGEGVNDVRKGACASHVSTMASFLKGAHVTINARAEEDVEPECIMEKVAKASGANYSFHKESGRFQDVGPQAPVGSVYQKTNAVSEIKRVGKDSFWAKAEKEEENRRLEEKRRAEEAQRQLEQERRERELREAARREQRYQEQGGEASPQRTWEQQQEVVSRNRNEQESAVHPREIFKQKERAMSTTSISSPQPGKLRSPFLQKQLTQPETHFGREPAAAISRPRADLPAEEPAPSTPPCLVQAEEEAVYEEPPEQETFYEQPPLVQQQGAGSEHIDHHIQGQGLSGQGLCARALYDYQAADDTEISFDPENLITGIEVIDEGWWRGYGPDGHFGMFPANYVELIE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UJU6 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| Y16 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| C127 | S-Nitrosylation | Uniprot | |
| K131 | Acetylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| K144 | Methylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| Y162 | Phosphorylation | Uniprot | |
| K164 | Methylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| K172 | Methylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K176 | Acetylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| T270 | Phosphorylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S275 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K280 | Acetylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| K288 | Acetylation | Uniprot | |
| K288 | Methylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| T291 | Phosphorylation | Uniprot | |
| T295 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| Y334 | Phosphorylation | P43403 (ZAP70) | Uniprot |
| Y344 | Phosphorylation | P43403 (ZAP70) | Uniprot |
研究背景
Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (By similarity). May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.
Degraded by caspases during apoptosis.
Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium. Cell projection>Ruffle. Cytoplasm>Cell cortex. Cytoplasm>Cytosol. Cell junction>Synapse. Perikaryon. Cell projection>Neuron projection. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Clathrin-coated vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Podosome. Early endosome. Cell projection>Dendrite. Cell junction>Synapse>Postsynaptic density.
Note: Associates with lamellipodial actin and membrane ruffles. Colocalizes with actin and cortactin at podosome dots and podosome rosettes.
Interacts with SHANK2, SHANK3 and SYN1. Interacts with FGD1 and DNM1. Interacts with ANKRD54. Interacts with COBL. Interacts with WASL and WIPF1 (By similarity). Interacts with MAP4K1 and PRAM1.
The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
Belongs to the ABP1 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.