DNAJB12 Antibody - #DF12598
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12598, RRID:AB_2845560.
折りたたみ／展開
DJ10; DJB12_HUMAN; DKFZp586B2023; DnaJ (Hsp40) homolog, subfamily B, member 12; DnaJ homolog subfamily B member 12; Dnajb12;
免疫原
- Q9NXW2 DJB12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVRALIESLNQKPQTAGDQPPPTDTTHATHRKAGGTDAPSANGEAGGESTKGYTAEQVAAVKRVKQCKDYYEILGVSRGASDEDLKKAYRRLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKSQAARHGHGHGDFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHRRVTDHLGVVYYVGDTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDTDMYHRAQKMGTPSCSRLSEVQASLHG
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NXW2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| Y158 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| Y345 | Phosphorylation | Uniprot | |
| Y352 | Phosphorylation | Uniprot |
研究背景
Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.
(Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection.
Endoplasmic reticulum membrane>Single-pass membrane protein. Nucleus membrane>Single-pass membrane protein.
Note: Localizes to the endoplasmic reticulum membrane (PubMed:21150129, PubMed:21148293, PubMed:24732912, PubMed:27916661). When overexpressed, forms membranous structures in the nucleus (PubMed:24732912).
Homodimer and homotetramer. Interacts (via J domain) with HSPA8/Hsc70. Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct.
Belongs to the DnaJ family. DNAJB12/DNAJB14 subfamily.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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