GSTA3 Antibody - #DF12624

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製品: GSTA3 Antibody
カタログ: DF12624
タンパク質の説明: Rabbit polyclonal antibody to GSTA3
アプリケーション: WB
反応性: Human, Mouse, Rat
分子量: 25 kDa; 25kD(Calculated).
ユニプロット: Q16772
RRID: AB_2845586

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
GSTA3 Antibody detects endogenous levels of total GSTA3.
RRID:
AB_2845586
引用形式: Affinity Biosciences Cat# DF12624, RRID:AB_2845586.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Glutathione S alkyltransferase A3; Glutathione S aralkyltransferase A3; Glutathione S aryltransferase A3; Glutathione S transferase A3 3; Glutathione S transferase A3; glutathione S transferase A3 subunit; Glutathione S transferase alpha 3; Glutathione S transferase alpha type (Ya); glutathione S transferase Yc 1; glutathione S transferase Yc1 subunit; Glutathione S-transferase A3; Glutathione S-transferase A3-3; Glutathione S-transferase Ya3; Glutathione S-transferase Yc; glutathione transferase A3 subunit; Glutathione transferase alpha 3; GST 2 2; GST AA; GST class alpha; GST class alpha member 3; GST class-alpha member 3; GST Yc1; GSTA 3; GSTA3 3; Gsta3; GSTA3-3; GSTA3_HUMAN; Gstyc; GTA 3; GTA3; MGC22232; S (hydroxyalkyl)glutathione lyase A3; Yc1;

免疫原

免疫原:
Uniprot:

Q16772(GSTA3_HUMAN) >>Visit HPA database.

遺伝子(ID):
GSTA3(2940)
タンパク質配列:
MAGKPKLHYFNGRGRMEPIRWLLAAAGVEFEEKFIGSAEDLGKLRNDGSLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYTEGMADLNEMILLLPLCRPEEKDAKIALIKEKTKSRYFPAFEKVLQSHGQDYLVGNKLSRADISLVELLYYVEELDSSLISNFPLLKALKTRISNLPTVKKFLQPGSPRKPPADAKALEEARKIFRF

PTMs - Q16772 基板として

Site PTM Type Enzyme
S49 Phosphorylation
Y74 Phosphorylation
S77 Phosphorylation
Y79 Phosphorylation
Y82 Phosphorylation
K84 Acetylation
K87 Acetylation
K125 Ubiquitination
S142 Phosphorylation
Y147 Phosphorylation
K152 Acetylation
S154 Phosphorylation
S189 Phosphorylation
T193 Phosphorylation
K195 Acetylation
K196 Acetylation
S202 Phosphorylation

研究背景

機能:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta(5)-androstene-3,17-dione and delta(5)-pregnene-3,20-dione, precursors to testosterone and progesterone, respectively.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer.

タンパク質ファミリー:

Belongs to the GST superfamily. Alpha family.

研究領域

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

参考文献

1). Long-Term Sleep Deprivation-Induced Myocardial Remodeling and Mitochondrial Dysfunction in Mice Were Attenuated by Lipoic Acid and N-Acetylcysteine. Pharmaceuticals (Basel, Switzerland), 2022 (PubMed: 36678548) [IF=4.6]

Application: WB    Species: Mouse    Sample: hearts

Figure 3. RNA sequencing revealed the Sirt1−Gsta3 axis is involved in CSD−regulated heart dysfunction. (A) RNA sequencing heat map clustered in hierarchy volcano map (B) shows up− or down−regulated genes in NC and CSD hearts. (C) Signal pathway analysis of the differential expression (DE) gene of RNA−seq shows the top 10 pathways related to CSD treatment. (D) Heat map clustered in hierarchy shows that Sirt1 of the Nicotinate and nicotinamide metabolism pathway (Left panel) and Gsta3 of the metabolic pathway were downregulated in the CSD heart. (E) Representative Western blot images and quantification of Sirt1 and Gsta3 in NC and CSD hearts. (F) Representative Western blot images and quantification of phosphorylation or total of AMPK and Akt in NC and CSD hearts.

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