IDH3A Antibody - #DF12641
| 製品: | IDH3A Antibody |
| カタログ: | DF12641 |
| タンパク質の説明: | Rabbit polyclonal antibody to IDH3A |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| 分子量: | 40 kDa; 40kD(Calculated). |
| ユニプロット: | P50213 |
| RRID: | AB_2845603 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12641, RRID:AB_2845603.
折りたたみ／展開
H IDH alpha; HIDH alpha; IDH3A; IDH3A_HUMAN; Isocitrate dehydrogenase (NAD+) alpha chain precursor; Isocitrate dehydrogenase [NAD] subunit alpha; Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial; Isocitrate dehydrogenase 3 (NAD+) alpha; Isocitrate dehydrogenase; Isocitric dehydrogenase; Isocitric dehydrogenase subunit alpha; mitochondrial; NAD; NAD(+)-specific ICDH subunit alpha; NAD(H) specific isocitrate dehydrogenase alpha subunit precursor; NAD+ specific ICDH; NADsubunit alpha mitochondrial precursor; OTTHUMP00000184906;
免疫原
- P50213 IDH3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P50213 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| R66 | Methylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| K77 | Acetylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| K90 | Acetylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| K177 | Acetylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| K214 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K343 | Acetylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot |
研究背景
Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
Mitochondrion.
Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
研究領域
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
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