MED12 Antibody - #DF12656
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12656, RRID:AB_2845618.
折りたたみ／展開
Activator recruited cofactor 240 kDa component; Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; CAGH45; HOPA; KIAA0192; MED12; MED12_HUMAN; Mediator complex subunit 12; Mediator of RNA polymerase II transcription subunit 12; OPA containing protein; OPA-containing protein; Thyroid hormone receptor associated protein complex 230 kDa component; Thyroid hormone receptor-associated protein complex 230 kDa component; TNRC11; Trap230; Trinucleotide repeat containing gene 11 protein; Trinucleotide repeat-containing gene 11 protein;
免疫原
- Q93074 MED12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q93074 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K32 | Ubiquitination | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| Y166 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| S635 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| K762 | Ubiquitination | Uniprot | |
| K773 | Ubiquitination | Uniprot | |
| K776 | Ubiquitination | Uniprot | |
| K780 | Ubiquitination | Uniprot | |
| K785 | Ubiquitination | Uniprot | |
| K814 | Ubiquitination | Uniprot | |
| K832 | Ubiquitination | Uniprot | |
| K988 | Ubiquitination | Uniprot | |
| K999 | Acetylation | Uniprot | |
| K999 | Ubiquitination | Uniprot | |
| K1001 | Ubiquitination | Uniprot | |
| K1181 | Ubiquitination | Uniprot | |
| K1194 | Ubiquitination | Uniprot | |
| S1258 | Phosphorylation | Uniprot | |
| S1269 | Phosphorylation | Uniprot | |
| K1298 | Ubiquitination | Uniprot | |
| K1414 | Ubiquitination | Uniprot | |
| K1434 | Ubiquitination | Uniprot | |
| K1445 | Ubiquitination | Uniprot | |
| K1453 | Ubiquitination | Uniprot | |
| K1471 | Ubiquitination | Uniprot | |
| K1524 | Ubiquitination | Uniprot | |
| K1534 | Ubiquitination | Uniprot | |
| K1619 | Ubiquitination | Uniprot | |
| K1622 | Ubiquitination | Uniprot | |
| K1634 | Ubiquitination | Uniprot | |
| K1643 | Ubiquitination | Uniprot | |
| K1661 | Ubiquitination | Uniprot | |
| K1664 | Ubiquitination | Uniprot | |
| K1673 | Ubiquitination | Uniprot | |
| K1674 | Ubiquitination | Uniprot | |
| K1682 | Ubiquitination | Uniprot | |
| K1761 | Acetylation | Uniprot | |
| K1761 | Ubiquitination | Uniprot | |
| S1778 | Phosphorylation | Uniprot | |
| T1779 | Phosphorylation | Uniprot | |
| K1798 | Acetylation | Uniprot | |
| K1798 | Ubiquitination | Uniprot | |
| S1838 | Phosphorylation | Uniprot | |
| R1854 | Methylation | Uniprot | |
| R1859 | Methylation | Uniprot | |
| R1862 | Methylation | Uniprot | |
| R1871 | Methylation | Uniprot | |
| R1899 | Methylation | Uniprot | |
| R1910 | Methylation | Uniprot | |
| R1912 | Methylation | Uniprot | |
| R1994 | Methylation | Uniprot | |
| R2015 | Methylation | Uniprot | |
| S2163 | Phosphorylation | Uniprot |
研究背景
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
Nucleus.
Ubiquitous.
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3.
Belongs to the Mediator complex subunit 12 family.
研究領域
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.