NSFL1C Antibody - #DF12677
| 製品: | NSFL1C Antibody |
| カタログ: | DF12677 |
| タンパク質の説明: | Rabbit polyclonal antibody to NSFL1C |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 41 kDa; 41kD(Calculated). |
| ユニプロット: | Q9UNZ2 |
| RRID: | AB_2845639 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12677, RRID:AB_2845639.
折りたたみ／展開
DJ776F14.1; MGC3347; NSF1C_HUMAN; NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; NSFL1C; OTTHUMP00000029972; OTTHUMP00000029974; p47; P47 protein; p97 cofactor p47; SHP1 homolog; UBX domain protein 2C; UBX domain-containing protein 2C; UBX1; UBXD10; UBXN2C;
免疫原
- Q9UNZ2 NSF1C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UNZ2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S68 | Phosphorylation | Uniprot | |
| T73 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| R93 | Methylation | Uniprot | |
| Y95 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K124 | Methylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K146 | Acetylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| K172 | Acetylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| R199 | Methylation | Uniprot | |
| R223 | Methylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K247 | Methylation | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| K251 | Acetylation | Uniprot | |
| K251 | Methylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| K356 | Ubiquitination | Uniprot |
研究背景
Reduces the ATPase activity of VCP (By similarity). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase. Also, regulates spindle orientation during mitosis.
Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis (By similarity).
Nucleus. Golgi apparatus>Golgi stack. Chromosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks. Localizes to centrosome during mitotic prophase and metaphase.
Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono-ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP (By similarity).
Belongs to the NSFL1C family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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