PGAM1 Antibody - #DF12693

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製品: PGAM1 Antibody
カタログ: DF12693
タンパク質の説明: Rabbit polyclonal antibody to PGAM1
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 29 kDa; 29kD(Calculated).
ユニプロット: P18669
RRID: AB_2845654

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
PGAM1 Antibody detects endogenous levels of total PGAM1.
RRID:
AB_2845654
引用形式: Affinity Biosciences Cat# DF12693, RRID:AB_2845654.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BPG dependent PGAM 1; BPG dependent PGAM1; BPG-dependent PGAM 1; OTTHUMP00000059414; PGAM 1; PGAM A; PGAM B; PGAM-B; PGAM1; PGAM1_HUMAN; PGAMA; PGAMB; Phosphoglycerate mutase 1; Phosphoglycerate mutase 1 brain; Phosphoglycerate mutase A; Phosphoglycerate mutase A nonmuscle form; Phosphoglycerate mutase isozyme B;

免疫原

免疫原:
Uniprot:

P18669(PGAM1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PGAM1(5223)
発現特異性:
P18669 PGAM1_HUMAN:

Expressed in the liver and brain. Not found in the muscle.

タンパク質配列:
MAAYKLVLIRHGESAWNLENRFSGWYDADLSPAGHEEAKRGGQALRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVYELDKNLKPIKPMQFLGDEETVRKAMEAVAAQGKAKK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18669 基板として

Site PTM Type Enzyme
K5 Acetylation
K5 Methylation
K5 Ubiquitination
H11 Phosphorylation
S14 Phosphorylation
R21 Methylation
S23 Phosphorylation Q16512 (PKN1) , Q13153 (PAK1)
Y26 Phosphorylation
S31 Phosphorylation
K39 Acetylation
K39 Ubiquitination
Y50 Phosphorylation
R90 Methylation
Y92 Phosphorylation
T96 Phosphorylation
K100 Acetylation
K100 Sumoylation
K100 Ubiquitination
K106 Acetylation
K106 Ubiquitination
K113 Acetylation
K113 Ubiquitination
S118 Phosphorylation Q13153 (PAK1) , Q16512 (PKN1)
Y119 Phosphorylation
Y133 Phosphorylation
S134 Phosphorylation
K138 Acetylation
K138 Ubiquitination
T146 Phosphorylation
S152 Phosphorylation
C153 S-Nitrosylation
S155 Phosphorylation
K157 Acetylation
K157 Ubiquitination
K176 Ubiquitination
S189 Phosphorylation
K195 Ubiquitination
K225 Ubiquitination
K228 Ubiquitination
T238 Phosphorylation
K241 Methylation
K241 Ubiquitination
K251 Acetylation
K251 Ubiquitination
K253 Acetylation
K253 Methylation
K254 Acetylation

研究背景

機能:

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

PTMs:

Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme.

組織特異性:

Expressed in the liver and brain. Not found in the muscle.

サブユニット構造:

Homodimer.

タンパク質ファミリー:

Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

研究領域

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

参考文献

1). Pimozide inhibits the growth of breast cancer cells by alleviating the Warburg effect through the P53 signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 35658233) [IF=7.5]

Application: WB    Species: Human    Sample: MCF-7 cells

Fig. 3. Pimozide inhibits PKM2 protein and mRNA in both MCF-7 and MDA-MB-231 cells in vitro. (A-B) Cells were treated with the indicated concentrations of Pimozide for 24 h, and the protein expression of glycolytic enzymes in MCF-7(A) and MDA-MB-231(B) cells were determined by Western blot analysis (left panel). Densitometry analysis was performed to assess the glycolytic enzymes protein expression (normalized to β-actin expression), PKM2 decreased significantly compared with untreated cells (right panel). (C-D) The mRNA expression of PKM2 in MCF-7(C) and MDA-MB-231(D) cells untreated or treated with Pimozide was determined by qRT-PCR. GAPDH was used as a control. Data represent mean ± SD from three biological replicates (*p < 0.05, **p < 0.01).
2). Proteomic analyses reveal higher levels of neutrophil activation in men than in women with systemic lupus erythematosus. Frontiers in Immunology, 2022 (PubMed: 35799787) [IF=7.3]

Application: WB    Species: Human    Sample:

Figure 6 Western Blot Validation of Proteins in Neutrophil Activation Networks. (A) Western blot analysis of BST2, PGAM1 and SERPINB10 in male SLE and female SLE. (B) Boxplot showing gray value of BST2 in male SLE and female SLE. (C) Boxplot showing gray value of PGAM1 in male SLE and female SLE. (D) Boxplot showing gray value of SERPINB10 in male SLE and female SLE.
3). Non-Esterified Fatty Acid-Induced Apoptosis in Bovine Granulosa Cells via ROS-Activated PI3K/AKT/FoxO1 Pathway. Antioxidants, 2023 (PubMed: 36829992) [IF=7.0]

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